Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division.
Extracellular lipidosomes containing lipid droplets and mitochondria are released during melanoma cell division.
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在黑色素瘤细胞分裂过程中释放含有脂滴和线粒体的细胞外核糖体。
DOI：
10.1186/s12964-024-01471-7
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发表时间：
2024-01-19
影响因子：
8.4
通讯作者：
Corbeil, Denis
中科院分区：
文献类型：
--
作者：
Karbanova, Jana;Deniz, Ilker A.;Wilsch-Braeuninger, Michaela;Couto, Rita Alexandra de Sousa;Fargeas, Christine A.;Santos, Mark F.;Lorico, Aurelio;Corbeil, Denis
黑色素瘤的发病率在全球范围内呈上升趋势。由于转移性黑色素瘤是高度侵袭性的，破译黑色素瘤细胞的所有生物学方面是很重要的。在此背景下，我们以前已经证明，转移性Femx-I黑色素瘤细胞释放小的(<；CD133)细胞外小泡( 150 nm)，称为外体和外体，其中包含干细胞(和癌症干细胞)抗原标记CD133。电动汽车在细胞间通讯中扮演着重要的角色，这可能会对周围组织的微环境产生影响。我们在这里报道了一种由FEMX-I细胞释放的新型大型CD133+EV。它们的大小从2到6微米不等，含有脂滴和线粒体。实时视频显微镜显示，这些EV起源于富含脂滴的细胞末端，在细胞分裂过程中没有完全回缩。一旦释放，它们就可以被其他细胞吸收。沉默CD133显著影响脂滴的细胞分布，并在核室周围重新定位。结果，含有脂滴的大EVS的形成受到严重影响。鉴于脂滴、线粒体和/或它们的复合体对细胞代谢的生化效应，这些从分裂的侵袭性黑色素瘤细胞中释放和摄取的新的大分子CD133+Ev可以同时影响供体细胞和受体细胞，从而影响黑色素瘤的生长和扩散。网上版载有补充材料，可在10.1186/s12964-024-01471-7查阅。
The incidence of melanoma is increasing worldwide. Since metastatic melanoma is highly aggressive, it is important to decipher all the biological aspects of melanoma cells. In this context, we have previously shown that metastatic FEMX-I melanoma cells release small (< 150 nm) extracellular vesicles (EVs) known as exosomes and ectosomes containing the stem (and cancer stem) cell antigenic marker CD133. EVs play an important role in intercellular communication, which could have a micro-environmental impact on surrounding tissues. We report here a new type of large CD133+ EVs released by FEMX-I cells. Their sizes range from 2 to 6 µm and they contain lipid droplets and mitochondria. Real-time video microscopy revealed that these EVs originate from the lipid droplet-enriched cell extremities that did not completely retract during the cell division process. Once released, they can be taken up by other cells. Silencing CD133 significantly affected the cellular distribution of lipid droplets, with a re-localization around the nuclear compartment. As a result, the formation of large EVs containing lipid droplets was severely compromised. Given the biochemical effect of lipid droplets and mitochondria and/or their complexes on cell metabolism, the release and uptake of these new large CD133+ EVs from dividing aggressive melanoma cells can influence both donor and recipient cells, and therefore impact melanoma growth and dissemination. The online version contains supplementary material available at 10.1186/s12964-024-01471-7.
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影响因子：
11.1
作者：
Bauer, Nicola;Wilsch-Braeuninger, Michaela;Karbanova, Jana;Fonseca, Ana-Violeta;Strauss, Doreen;Freund, Daniel;Thiele, Christoph;Huttner, Wieland B.;Bornhaeuser, Martin;Corbeil, Denis
通讯作者：
Corbeil, Denis
影响因子：
4.3
作者：
Huang, Jin;Jin, Yi;Yu, Ziwei;Zhang, Junzhi;Jian, Wu;Ren, Zhuqing
通讯作者：
Ren, Zhuqing
CDK1-CYCLIN-B1诱导的Kindlin降解在有丝分裂进入时脱离了局灶性粘附。
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
7.5
作者：
Al Dhaybi, Rola;Sartelet, Herve;Kokta, Victor
通讯作者：
Kokta, Victor
影响因子：
3.7
作者：
Bedrosian, I;Faries, MB;Czerniecki, BJ
通讯作者：
Czerniecki, BJ