The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.
The TcI and TcII Trypanosoma cruzi experimental infections induce distinct immune responses and cardiac fibrosis in dogs.
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DOI：
10.1590/0074-02760140208
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发表时间：
2014-12
影响因子：
2.8
通讯作者：
Carneiro CM
中科院分区：
文献类型：
--
作者：
Duz AL;Vieira PM;Roatt BM;Aguiar-Soares RD;Cardoso JM;Oliveira FC;Reis LE;Tafuri WL;Veloso VM;Reis AB;Carneiro CM
克氏锥虫感染可能是由具有不同离散分型单位(DTU)的不同菌株引起的，这些菌株可导致各种临床形式的慢性恰加斯病。本研究用不同的TcI和TcII DTU的哥伦比亚株(Col)和Y株，对实验感染犬的免疫应答和心脏损害进行了评价。在感染Col株期间，观察到丙氨酸氨基转移酶、红细胞、红细胞压积和血红蛋白水平升高。此外，从外周血中分离的CD8+T淋巴细胞产生更高水平的白介素4。后者表明，在急性期，循环单核细胞可能不会注意到Col株的感染。慢性期，右心房炎性细胞数量明显增多。反之，Y株感染可导致白细胞减少、血小板减少、CD4+/CD8+T淋巴细胞比例倒置和单核细胞数量改变。Y株能刺激CD_4~+、CD_8~+T细胞产生干扰素-γ，CD_8~+T细胞产生IL-4。在慢性期，观察到明显的心脏炎症和纤维化，表明不同的DTU菌株与宿主的相互作用不同。
Trypanosoma cruzi infection may be caused by different strains with distinct discrete typing units (DTUs) that can result in variable clinical forms of chronic Chagas disease. The present study evaluates the immune response and cardiac lesions in dogs experimentally infected with different T. cruzi strains with distinct DTUs, namely, the Colombian (Col) and Y strains of TcI and TcII DTU, respectively. During infection with the Col strain, increased levels of alanine aminotransferase, erythrocytes, haematocrit and haemoglobin were observed. In addition, CD8+ T-lymphocytes isolated from the peripheral blood produced higher levels of interleukin (IL)-4. The latter suggests that during the acute phase, infection with the Col strain may remain unnoticed by circulating mononuclear cells. In the chronic phase, a significant increase in the number of inflammatory cells was detected in the right atrium. Conversely, infection with the Y strain led to leucopoenia, thrombopoenia, inversion of the ratio of CD4+/CD8+ T-lymphocytes and alterations in monocyte number. The Y strain stimulated the production of interferon-γ by CD4+ and CD8+ T-lymphocytes and IL-4 by CD8+ T-cells. In the chronic phase, significant heart inflammation and fibrosis were observed, demonstrating that strains of different DTUs interact differently with the host.
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影响因子：
3.7
作者：
Vieira PM;Francisco AF;Machado EM;Nogueira NC;Fonseca Kda S;Reis AB;Teixeira-Carvalho A;Martins-Filho OA;Tafuri WL;Carneiro CM
通讯作者：
Carneiro CM
影响因子：
2.6
作者：
de Almeida Leal, Gleisiane Gomes;Roatt, Bruno Mendes;Reis, Alexandre Barbosa
通讯作者：
Reis, Alexandre Barbosa
影响因子：
3.2
作者：
Aguiar-Soares, Rodrigo Dian de Oliveira;Roatt, Bruno Mendes;Reis, Alexandre Barbosa
通讯作者：
Reis, Alexandre Barbosa
影响因子：
1.6
作者：
Comazzi, S;Pieralisi, C;Bertazzolo, W
通讯作者：
Bertazzolo, W
影响因子：
3.8
作者：
de Melo, Adriene Siqueira;Barros de Lorena, Virginia Maria;Gomes, Yara de Miranda
通讯作者：
Gomes, Yara de Miranda