Effects of Maternal Low-Energy Diet during Gestation on Intestinal Morphology, Disaccharidase Activity, and Immune Response to Lipopolysaccharide Challenge in Pig Offspring.
Effects of Maternal Low-Energy Diet during Gestation on Intestinal Morphology, Disaccharidase Activity, and Immune Response to Lipopolysaccharide Challenge in Pig Offspring.
复制标题
妊娠期母体低能量饮食对子代猪肠道形态、二糖酶活性和脂多糖激发免疫反应的影响
作者：
Chen Y;Mou D;Hu L;Zhen J;Che L;Fang Z;Xu S;Lin Y;Feng B;Li J;Wu D
母体妊娠期营养状况与子代肠道发育和免疫力有关。本研究以仔猪为模型，探讨母体能量对仔猪肠道消化吸收功能的影响;探讨母体能量对脂多糖（LPS）攻击仔猪免疫应答的调节作用及其机制。将36头9胎次的长白×约克郡母猪（初体重233.56 ± 2.77 kg）交配后随机分为对照组（CON组）和低能量组（LED组）。CON的营养水平基于国家研究理事会（NRC，2012）的营养推荐，并且含有3.40 MCal可消化能（DE）/kg饮食和7.3%粗蛋白;而LED含有3.00 MCal DE/kg饮食。从妊娠第1天至分娩引入饮食处理。在出生时和断奶时（出生后第28天）从猪的后代中收集肠道样品。在断奶时，来自对照组和LED组的雄性猪腹腔内注射LPS（50 μg/kg体重）或生理盐水（n = 6），并在注射后4 h处死以收集血液、肠和粪便样品用于生化分析。结果表明，母鼠饲喂低剂量日粮可显著降低仔鼠的体重、小肠重量和空回肠绒毛高度与隐窝深度的比值。此外，母畜日粮中添加低剂量的低剂量维生素D，还显著降低了新生仔猪肠道中乳糖酶和蔗糖酶的活性，以及断奶仔猪肠道中蔗糖酶和麦芽糖酶的活性。此外，母畜LED显著增加新生仔猪回肠IL-6和TNF-α mRNA相对表达量。血浆IL-1β浓度和大肠埃希氏菌数量受母体饮食（p < 0.05）和LPS攻击（p < 0.001）的影响。母鼠LED显著增加LPS刺激后断奶仔猪回肠TLR-4、IL-1β和NF-κB的mRNA相对表达量，降低ZO-1的mRNA相对表达量（p < 0.05）。由此可见，降低能量摄入可抑制仔猪肠道消化吸收功能，增加断奶仔猪对LPS攻击的易感性。
Maternal nutrition during gestation is involved in the offspring’s intestinal development and immunity. The aim of this study was to (1) determine the effects of maternal energy on intestinal digestion and absorption function in offspring, using pigs as a model; and (2) to evaluate the potential effect and mechanisms of maternal energy in modulating immune responses of lipopolysaccharide (LPS)-challenged piglets. After mating, thirty-six nine-parity sows (Landrace × Yorkshire), body weight (BW) (initial body weight 233.56 ± 2.77 kg) were allocated to two dietary treatment groups; a control diet (CON) group and a low-energy diet (LED) group. The nutrient levels of the CON were based on the nutrient recommendations by the National Research Council (NRC, 2012), and contained 3.40 MCal digestible energy (DE)/kg diet and 7.3% crude protein; while the LED contained 3.00 MCal DE/kg diet. The dietary treatments were introduced from day 1 of gestation to farrowing. Intestine samples were collected from the pigs’ offspring at birth, and at weaning (day 28 post-birth). At weaning, male pigs from control and LED groups were intraperitoneally injected with LPS (50 μg/kg body weight) or saline (n = 6), and sacrificed at 4 h post-injection to collect blood, intestine and digesta samples for biochemical analysis. The results indicated that the maternal LED markedly decreased the BW, small intestinal weight, and the ratio of jejunum and ileum villus height to crypt depth in the offspring. Moreover, the activities of lactase and sucrase in newborn piglets’ intestine, and sucrase and maltase in weaning piglet intestine were markedly decreased by the maternal LED. In addition, maternal LED significantly increased the mRNA relative expression of ileal IL-6 and TNF-α in newborn piglets. Plasma IL-1β concentration and colonic Escherichia coli amount were affected by maternal diet (p < 0.05) and LPS challenge (p < 0.001). Maternal LED significant increased the mRNA relative expression of ileal TLR-4, IL-1β and NF-κB as well as decreased ZO-1 in weaning pigs after LPS challenge (p < 0.05). In conclusion, decreasing energy intake could suppress the offspring’s intestinal digestion and absorption function, and increase the susceptibility of weaning piglets to LPS challenge.
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影响因子：
64.8
作者：
Fukuda, Shinji;Toh, Hidehiro;Ohno, Hiroshi
通讯作者：
Ohno, Hiroshi
影响因子：
4.2
作者：
D'Inca, Romain;Kloareg, Maela;Le Huerou-Luron, Isabelle
通讯作者：
Le Huerou-Luron, Isabelle
DOI：
10.1016/s1877-1823(09)70118-3
发表时间：
2002-01-01
期刊：
BIOLOGY OF THE INTESTINE IN GROWING ANIMALS
影响因子：
--
作者：
Baintner, K
通讯作者：
Baintner, K
影响因子：
24.5
作者：
Cani PD;Possemiers S;Van de Wiele T;Guiot Y;Everard A;Rottier O;Geurts L;Naslain D;Neyrinck A;Lambert DM;Muccioli GG;Delzenne NM
通讯作者：
Delzenne NM
影响因子：
2.5
作者：
Buccigrossi, V.;Giannattasio, A.;Guarino, A.
通讯作者：
Guarino, A.