Methylene blue covalently loaded polyacrylamide nanoparticles for enhanced tumor-targeted photodynamic therapy.
Methylene blue covalently loaded polyacrylamide nanoparticles for enhanced tumor-targeted photodynamic therapy.
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DOI：
10.1039/c1pp05022b
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Kopelman R
中科院分区：
文献类型：
--
作者：
Qin M;Hah HJ;Kim G;Nie G;Lee YE;Kopelman R
使用靶向纳米颗粒（NP）作为装载光敏剂的平台，使得光敏剂能够在肿瘤区域中选择性积累，同时保持其光动力治疗（PDT）的有效性。在这里，两种新的类型的亚甲蓝（MB）共轭聚丙烯酰胺（PAA）纳米粒子，MBI-PAA纳米粒子和MBII-PAA纳米粒子，基于两个单独的MB衍生物，开发的PDT。这种与NP的共价缀合（i）改善MB的负载，（ii）防止MB从NP的任何浸出，和（iii）保护MB免受生物环境中酶的影响。MB到这两种NP中的负载由输入量控制，从而产生具有最佳单线态氧产生的浓度。对于每种MB-NP，发现在MB负载量为约11 nmol mg-1时单线态氧产量最高。在附着F3肽基团之后，为了靶向，这些NP中的每一个在体外被MDA-MB-435肿瘤细胞选择性地摄取。PDT测试表明，两种靶向NP在照射后均导致有效的肿瘤细胞杀伤，而不引起暗毒性。
The use of targeted nanoparticles (NPs) as a platform for loading photosensitizers enables selective accumulation of the photosensitizers in the tumor area, while maintaining their photodynamic therapy (PDT) effectiveness. Here two novel kinds of methylene blue (MB)-conjugated polyacrylamide (PAA) nanoparticles, MBI-PAA NPs and MBII-PAA NPs, based on two separate MB derivatives, are developed for PDT. This covalent conjugation with the NPs (i) improves the loading of MB, (ii) prevents any leaching of MB from the NPs and (iii) protects the MB from the effects of enzymes in the biological environment. The loading of MB into these two kinds of NPs was controlled by the input amount, resulting in concentrations with optimal singlet oxygen production. For each of the MB-NPs, the highest singlet oxygen production was found for an MB loading of around 11 nmol mg−1. After attachment of F3 peptide groups, for targeting, each of these NPs was taken up, selectively, by MDA-MB-435 tumor cells, in vitro. PDT tests demonstrated that both kinds of targeted NPs resulted in effective tumor cell kill, following illumination, while not causing dark toxicity.
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影响因子：
2.7
作者：
Kopelman, R;Koo, YEL;Ross, BD
通讯作者：
Ross, BD
影响因子：
4.6
作者：
Hah, Hoe Jin;Kim, Gwangseong;Kopelman, Raoul
通讯作者：
Kopelman, Raoul
影响因子：
8.4
作者：
Moreno, MJ;Monson, E;Kopelman, R
通讯作者：
Kopelman, R
影响因子：
3.3
作者：
Patil, K;Pawar, R;Talap, P
通讯作者：
Talap, P
DOI：
10.1152/ajplung.1995.269.1.l78
发表时间：
1995-07-01
影响因子：
4.9
作者：
BONGARD, RD;MERKER, MP;DAWSON, CA
通讯作者：
DAWSON, CA