When two cells are better than one: specialized stellate cells provide a privileged route for uniquely rapid water flux in Drosophila renal tubule
When two cells are better than one: specialized stellate cells provide a privileged route for uniquely rapid water flux in Drosophila renal tubule
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当两个细胞比一个细胞更好时：特化的星状细胞为果蝇肾小管中独特的快速水通量提供了一条特权途径
DOI：
10.1101/763664
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Cabrero P
中科院分区：
文献类型：
--
作者：
Cabrero P
昆虫非常成功，这在一定程度上得益于出色的渗透调节能力。在每个细胞的基础上，肾(马氏管)可以比任何其他上皮细胞更快地分泌液体，但这些显著的水通量的途径尚未建立。在果蝇的肾脏组织中，我们发现了4个主要内源性蛋白家族的成员：水孔蛋白水滴蛋白和水孔蛋白，以及水甘油蛋白Eglp2和Eglp4。根据它们的结构和在Xenopus卵母细胞中表达这些蛋白的转运功能可以预测，Drop、Prip和Eglp2具有显著的特异性透水性，而Eglp2和Eglp4对甘油和尿素具有很高的通透性。这些基因中的任何一个的敲除都会影响小管的性能，导致激素诱导的液体分泌受损。果蝇小管有两种主要的分泌细胞类型：活跃的阳离子转运主细胞和小星状细胞，前者定位于相对的质膜，后者是氯分流电导的位置，这些水通道蛋白定位于相对的质膜。这提出了一种模型，在该模型中，阳离子由主细胞泵送，导致氯化物跟随星状细胞，以平衡电荷。结果，渗透压作用的水通过星状细胞。与该模型一致的是，激肽利尿肽刺激后，荧光标记的葡聚糖(膜透性的活体标志物)被困在星状细胞的基底膜内，证实了这些细胞提供了跨上皮水通量的主要途径。上皮水分运输的这些成分在空间上的分离可能有助于解释高等昆虫独特的成功。意义陈述微小的昆虫肾脏(马氏管)可以以无与伦比的速度运输液体，这表明了独特的专门化。在这里，我们表明，主要固有蛋白(MIP)的战略分配到极化小管内的特定细胞允许从氯化物和水电导中分离代谢强烈的活性阳离子运输。这种形体计划至少对许多高等昆虫是通用的，为昆虫纲的独特成功提供了线索。
Insects are highly successful, in part through an excellent ability to osmoregulate. The renal (Malpighian) tubules can secrete fluid faster on a per-cell basis than any other epithelium, but the route for these remarkable water fluxes has not been established. InDrosophila melanogaster, we show that 4 members of the Major Intrinsic Protein family are expressed at very high level in the fly renal tissue; the aquaporins Drip and Prip, and the aquaglyceroporins Eglp2 and Eglp4. As predicted from their structure and by their transport function by expressing these proteins inXenopusoocytes, Drip, Prip and Eglp2 show significant and specific water permeability, whereas Eglp2 and Eglp4 show very high permeability to glycerol and urea. Knockdowns of any of these genes impacts tubule performance resulting in impaired hormone-induced fluid secretion. TheDrosophilatubule has two main secretory cell types: active cation-transporting principal cells with the aquaglyceroporins localize to opposite plasma membranes and small stellate cells, the site of the chloride shunt conductance, with these aquaporins localising to opposite plasma membranes. This suggests a model in which cations are pumped by the principal cells, causing chloride to follow through the stellate cells in order to balance the charge. As a consequence, osmotically obliged water follows through the stellate cells. Consistent with this model, fluorescently labelled dextran, anin vivomarker of membrane water permeability, is trapped in the basal infoldings of the stellate cells after kinin diuretic peptide stimulation, confirming that these cells provide the major route for transepithelial water flux. The spatial segregation of these components of epithelial water transport may help to explain the unique success of the higher insects.Significance statementThe tiny insect renal (Malpighian) tubule can transport fluid at unparalleled speed, suggesting unique specialisations. Here we show that strategic allocation of Major Intrinsic Proteins (MIPs) to specific cells within the polarized tubule allow the separation of metabolically intense active cation transport from chloride and water conductance. This body plan is general to at least many higher insects, providing a clue to the unique success of the class Insecta.
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DOI：
10.1242/dev.088989
发表时间：
2013-03
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Denholm B;Hu N;Fauquier T;Caubit X;Fasano L;Skaer H
通讯作者：
Skaer H
K :Cl- 共转运和 Na /K -ATP 酶对果蝇马氏管基底外侧离子转运的贡献。
影响因子：
2.8
作者：
Stuart M. Linton;Michael J. O'Donnell
通讯作者：
Michael J. O'Donnell
DOI：
10.1152/ajpregu.00148.2015
发表时间：
2015-07
期刊：
American journal of physiology. Regulatory, integrative and comparative physiology
影响因子：
--
作者：
Yipin Wu;M. Baum;Chou-Long Huang;Aylin R. Rodan
通讯作者：
Yipin Wu;M. Baum;Chou-Long Huang;Aylin R. Rodan
差异凝胶电泳和转基因线粒体钙报告基因展示了线粒体钙控制中的时空过滤*
影响因子：
4.8
作者：
Selim Terhzaz;T. Southall;K. Lilley;L. Kean;A. Allan;S. Davies;J. Dow
通讯作者：
J. Dow
影响因子：
2.8
作者：
Misyura, Lidiya;Yerushalmi, Gil Y.;Donini, Andrew
通讯作者：
Donini, Andrew