The tiptop/teashirt genes regulate cell differentiation and renal physiology in Drosophila.
The tiptop/teashirt genes regulate cell differentiation and renal physiology in Drosophila.
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DOI：
10.1242/dev.088989
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发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Skaer H
中科院分区：
文献类型：
--
作者：
Denholm B;Hu N;Fauquier T;Caubit X;Fasano L;Skaer H
器官的生理活动是由它们所包含的不同细胞类型之间的相互作用所支撑的。然而，对器官发育过程中细胞分化模式的遗传控制知之甚少。我们发现，保守的Teashirt转录因子是决定性的分化的一个子集的分泌细胞，星状细胞，在果蝇肾小管。Teashirt控制水通道Drip、氯离子传导通道CLC-a和白细胞激肽受体（LKR）的表达，所有这些都是促分化的星状细胞，并且是初级尿产生和对利尿刺激的反应性所需的。在变态过程中，Teashirt还控制着细胞形态的戏剧性转变，从立方形到epperial星状。teashirt与cut相互作用，cut编码一种转录因子，该转录因子是初级主要分泌细胞分化的基础，建立了一个相互的负反馈环，确保两种细胞类型的完全分化。紧身衣的丢失导致无效的尿液产生，体内平衡的失败和过早死亡。通常不在幼虫或成体星状细胞中表达的teashirt paradipoptop的星状细胞特异性表达几乎完全挽救了teashirt从星状细胞的表达损失。我们证明保守的家庭的顶/teashirt基因在低等昆虫的表达和建立保守的Teashirt转录因子在小鼠胚胎肾的目标。
The physiological activities of organs are underpinned by an interplay between the distinct cell types they contain. However, little is known about the genetic control of patterned cell differentiation during organ development. We show that the conserved Teashirt transcription factors are decisive for the differentiation of a subset of secretory cells, stellate cells, in Drosophila melanogaster renal tubules. Teashirt controls the expression of the water channel Drip, the chloride conductance channel CLC-a and the Leukokinin receptor (LKR), all of which characterise differentiated stellate cells and are required for primary urine production and responsiveness to diuretic stimuli. Teashirt also controls a dramatic transformation in cell morphology, from cuboidal to the eponymous stellate shape, during metamorphosis. teashirt interacts with cut, which encodes a transcription factor that underlies the differentiation of the primary, principal secretory cells, establishing a reciprocal negative-feedback loop that ensures the full differentiation of both cell types. Loss of teashirt leads to ineffective urine production, failure of homeostasis and premature lethality. Stellate cell-specific expression of the teashirt paralogue tiptop, which is not normally expressed in larval or adult stellate cells, almost completely rescues teashirt loss of expression from stellate cells. We demonstrate conservation in the expression of the family of tiptop/teashirt genes in lower insects and establish conservation in the targets of Teashirt transcription factors in mouse embryonic kidney.
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