Existence of various human parvovirus B19 genotypes in Chinese plasma pools: genotype 1, genotype 3, putative intergenotypic recombinant variants and new genotypes.
Existence of various human parvovirus B19 genotypes in Chinese plasma pools: genotype 1, genotype 3, putative intergenotypic recombinant variants and new genotypes.
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中国血浆库中存在多种人类细小病毒 B19 基因型：基因型 1、基因型 3、推定的基因型间重组变体和新基因型
DOI：
10.1186/s12985-016-0611-6
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发表时间：
2016-09-17
期刊：
影响因子：
4.8
通讯作者：
Zhang J
中科院分区：
文献类型：
--
作者：
Jia J;Ma Y;Zhao X;Huangfu C;Zhong Y;Fang C;Fan R;Lv M;Zhang J
人细小病毒B19（B19 V）是血液和血浆衍生药品的常见污染物。已经鉴定了三种不同的B19 V基因型。三种B19 V基因型的分布已在不同地区或国家进行了研究。然而，在中国，关于不同B19 V基因型存在的数据有限。分析了从3家中国血液制品生产商采集的118份B19 V-DNA阳性源血浆池样本。采用巢式PCR技术扩增B19 V亚基因组NS 1/VP 1u连接区。然后克隆这些扩增产物并随后测序。对于基因分型，基于NS 1/VP 1独特区域构建它们的系统发育推断。然后分析和鉴定推定的重组事件。118个B19 V基因序列的系统发育分析表明，1a型占61.86%，1b型占10.17%，3b型占17.80%。本研究中获得的所有基因型3b序列与B19 V菌株D91.1分组为一个特定的密切相关的簇。鉴定了4个1a/3b重组体和5个新的非典型B19 V变异体，没有重组事件。在中国流行的B19 V至少有3种亚型（1a、1b和3b）。此外，还鉴定了推定的B19 V 1a/3b重组体和未分类菌株。这种重组和未分类的菌株可能有助于B19 V的遗传多样性，从而使B19 V感染诊断和NAT筛选复杂化。需要进一步的研究来阐明重组和未分类菌株的生物学意义。本文的在线版本（doi：10.1186/s12985-016-0611-6）包含补充材料，可供授权用户使用。
Human parvovirus B19 (B19V) is a frequent contaminant of blood and plasma-derived medicinal products. Three distinct genotypes of B19V have been identified. The distribution of the three B19V genotypes has been investigated in various regions or countries. However, in China, data on the existence of different B19V genotypes are limited. One hundred and eighteen B19V-DNA positive source plasma pool samples collected from three Chinese blood products manufacturers were analyzed. The subgenomic NS1/VP1u region junction of B19V was amplified by nested PCR. These amplified products were then cloned and subsequently sequenced. For genotyping, their phylogenetic inferences were constructed based on the NS1/VP1-unique region. Then putative recombination events were analyzed and identified. Phylogenetic analysis of 118 B19V sequences attributed 61.86 % to genotype 1a, 10.17 % to genotype 1b, and 17.80 % to genotype 3b. All the genotype 3b sequences obtained in this study grouped as a specific, closely related cluster with B19V strain D91.1. Four 1a/3b recombinants and 5 new atypical B19V variants with no recombination events were identified. There were at least 3 subtypes (1a, 1b and 3b) of B19V circulating in China. Furthermore, putative B19V 1a/3b recombinants and unclassified strains were identified as well. Such recombinant and unclassified strains may contribute to the genetic diversity of B19V and consequently complicate the B19V infection diagnosis and NAT screening. Further studies will be required to elucidate the biological significance of the recombinant and unclassified strains. The online version of this article (doi:10.1186/s12985-016-0611-6) contains supplementary material, which is available to authorized users.
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通讯作者：
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