Circular RNA hsa_circ_0001306 Functions as a Competing Endogenous RNA to Regulate FBXW7 Expression by Sponging miR-527 in Hepatocellular Carcinoma.
Circular RNA hsa_circ_0001306 Functions as a Competing Endogenous RNA to Regulate FBXW7 Expression by Sponging miR-527 in Hepatocellular Carcinoma.
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环状 RNA hsa_circ_0001306 作为竞争性内源 RNA 通过海绵 miR-527 在肝细胞癌中调节 FBXW7 表达
DOI：
10.7150/jca.61381
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发表时间：
2021
影响因子：
3.9
通讯作者：
Xue X
中科院分区：
文献类型：
--
作者：
Wu Y;Fan T;Zhao Y;Hu R;Yan D;Sun D;Gao L;Qin L;Xue X
肝细胞癌（HCC）是世界范围内最常见的癌症类型之一。已报道环状RNA（circRNA）调节许多类型的癌症，包括HCC。本研究的目的是研究hsa_circ_0001306在肝癌中的潜在作用。首先，通过高通量RNA测序鉴定hsa_circ_0001306的下调，并通过qRT-PCR进一步验证。其次，研究hsa_circ_0001306对肝癌细胞增殖、侵袭、细胞周期的影响。最后，我们使用动物模型来验证体外实验结果。hsa_circ_0001306的表达与肿瘤大小密切相关。hsa_circ_0001306的敲低可下调miR-527的靶点F-box and WD repeat domain containing 7（FBXW 7），从而促进HCC细胞增殖和侵袭。此外，hsa_circ_0001306 siRNA增加HCC肿瘤的增殖率。机制研究表明，hsa_circ_0001306充当miR-527的ceRNA，这导致其内源性靶标FBXW 7的减少。Hsa_circ_001306在HCC中显著下调，hsa_circ_0001306/miR-527/FBXW 7轴在HCC进展中起重要作用。
Hepatocellular carcinoma (HCC) is one of the most common types of cancer worldwide. Circular RNAs (circRNAs) have been reported to regulate many types of cancers, including HCC. The purpose of this study was to investigate the potential roles of hsa_circ_0001306 in HCC. Firstly, the downregulation of hsa_circ_0001306 was identified by high‑throughput RNA sequencing and further verified by qRT-PCR. Secondly, we evaluated the effects of hsa_circ_0001306 on HCC cell proliferation, invasion, cell cycle. Finally, we used an animal model to validate the in vitro experimental results. The expression of hsa_circ_0001306 was closely related to tumor size. Knockdown of hsa_circ_0001306 could downregulate F-box and WD repeat domain containing 7(FBXW7), a target of miR-527, thereby promoting HCC cell proliferation and invasion. Furthermore, hsa_circ_0001306 siRNA increased the multiplication rate of HCC tumors. Mechanistic studies indicated that hsa_circ_0001306 acts as a ceRNA for miR-527, which resulted in the reduction of its endogenous target, FBXW7. Hsa_circ_001306 is significantly downregulated in HCC, and the hsa_circ_0001306/miR-527/FBXW7 axis plays an important role in HCC progression.
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DOI：
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