Changes in composition and levels of hemolymph proteins during metamorphosis of Manduca sexta.
Changes in composition and levels of hemolymph proteins during metamorphosis of Manduca sexta.
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DOI：
10.1016/j.ibmb.2020.103489
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发表时间：
2020-12
影响因子：
3.8
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
Cao X;Wang Y;Rogers J;Hartson S;Kanost MR;Jiang H
烟草天蛾（Manducasexta）是鳞翅目昆虫生化过程研究的模式昆虫。它的一些幼虫血淋巴蛋白得到了很好的研究，2016年对幼虫血浆蛋白进行了详细的蛋白质组学分析，揭示了与转录组数据的相关性，免疫复合物的形成以及血淋巴中免疫信号系统的构成等特征。目前还不清楚这些蛋白质的组成在其他发育阶段可能会发生什么变化。本文报道了鸡包皮、第4天和第13天的蛹和青年的无细胞血淋巴的蛋白质组。在鉴定的1，824种蛋白质中，907种具有信号肽，410种与免疫有关。例如，贮存蛋白、脂蛋白和卵黄蛋白原丰度的急剧变化反映了包皮、脓疱和成虫之间的生理差异。相当多的缺乏信号肽的蛋白质存在于后期pupelia，这表明血浆中含有相对低浓度的细胞内成分释放的重塑组织在变态。检测到的防御蛋白包括43个丝氨酸蛋白酶和11个丝氨酸蛋白酶同源物。这些蛋白质中的一些是在摄食幼虫中发现的细胞外免疫信号网络的成员，而其他蛋白质可能发挥额外的作用，从而在以后的生命阶段赋予新的功能。总之，本研究中揭示的蛋白质及其水平，以及它们的转录组数据，有望刺激对游走幼虫、蛹和成虫的体液免疫和其他生理系统的集中探索。Sexta并为其他全变态昆虫的功能和比较基因组研究提供了线索。
The tobacco hornworm, Manduca sexta, is a lepidopteran model species widely used to study insect biochemical processes. Some of its larval hemolymph proteins are well studied, and a detailed proteomic analysis of larval plasma proteins became available in 2016, revealing features such as correlation with transcriptome data, formation of immune complexes, and constitution of an immune signaling system in hemolymph. It is unclear how the composition of these proteins may change in other developmental stages. In this paper, we report the proteomes of cell-free hemolymph from prepupae, pupae on day 4 and day 13, and young adults. Of the 1,824 proteins identified, 907 have a signal peptide and 410 are related to immunity. Drastic changes in abundance of the storage proteins, lipophorins and vitellogenin, for instance, reflect physiological differences among prepupae, pupae, and adults. Considerably more proteins lacking signal peptide are present in the late pupae, suggesting that plasma contains relatively low concentrations of intracellular components released from remodeling tissues during metamorphosis. The defense proteins detected include 43 serine proteases and 11 serine protease homologs. Some of these proteins are members of the extracellular immune signaling network found in feeding larvae, and others may play additional roles and hence confer new features in the later life stages. In summary, the proteins and their levels revealed in this study, together with their transcriptome data, are expected to stimulate focused explorations of humoral immunity and other physiological systems in wandering larvae, pupae, and adults of M. sexta and shed light upon functional and comparative genomic research in other holometabolous insects.
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影响因子：
2.9
作者：
He X;Cao X;He Y;Bhattarai K;Rogers J;Hartson S;Jiang H
通讯作者：
Jiang H
影响因子：
16
作者：
Issa N;Guillaumot N;Lauret E;Matt N;Schaeffer-Reiss C;Van Dorsselaer A;Reichhart JM;Veillard F
通讯作者：
Veillard F
影响因子：
3.8
作者：
Cao X;He Y;Hu Y;Zhang X;Wang Y;Zou Z;Chen Y;Blissard GW;Kanost MR;Jiang H
通讯作者：
Jiang H
影响因子：
23.8
作者：
Arrese EL;Soulages JL
通讯作者：
Soulages JL
影响因子：
3.4
作者：
DUNN, PE;DRAKE, DR
通讯作者：
DRAKE, DR