Hemolymph proteins of Anopheles gambiae larvae infected by Escherichia coli.
Hemolymph proteins of Anopheles gambiae larvae infected by Escherichia coli.
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DOI：
10.1016/j.dci.2017.04.009
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发表时间：
2017-09
影响因子：
2.9
通讯作者：
Jiang H
中科院分区：
文献类型：
--
作者：
He X;Cao X;He Y;Bhattarai K;Rogers J;Hartson S;Jiang H
冈比亚按蚊是人类疟疾的主要传播媒介，其免疫系统在一定程度上决定了摄入寄生虫的命运。血淋巴中的蛋白质、血细胞和脂肪体是该系统的重要组成部分，介导体液和细胞防御。在这里，我们用凝胶-LC-MS方法评估了水刺激和大肠杆菌刺激的蚊子幼虫血淋巴蛋白质组的差异。在鉴定的1,756种蛋白质中，603种含有信号肽，但在定量的基础上占总蛋白质数量的三分之二。序列同源性搜索表明，1,756个序列中有233个可能与防御有关。总体而言，我们没有检测到对照和诱导血浆样本在蛋白质数量或水平方面的实质性差异。凝胶切片中的蛋白质分布表明翻译后修饰(如蛋白质降解)和形成丝氨酸蛋白酶复合体和高MR免疫复合体。基于25种最丰富的蛋白质，我们进一步认为幼虫血淋巴的主要功能是储存、运输和免疫。总之，这项研究首次提供了蚊子幼虫血淋巴蛋白的组成、水平和可能功能的数据，反映了在对抗大肠杆菌感染的过程中发生的复杂变化。
Anopheles gambiae is a major vector of human malaria and its immune system in part determines the fate of ingested parasites. Proteins, hemocytes and fat body in hemolymph are critical components of this system, mediating both humoral and cellular defenses. Here we assessed differences in the hemolymph proteomes of water- and E. coli-pricked mosquito larvae by a gel-LC-MS approach. Among the 1,756 proteins identified, 603 contained a signal peptide but accounted for two-third of the total protein amount on the quantitative basis. The sequence homology search indicated that 233 of the 1,756 may be related to defense. In general, we did not detect substantial differences between the control and induced plasma samples in terms of protein numbers or levels. Protein distributions in the gel slices suggested post-translational modifications (e.g. proteolysis) and formation of serpin-protease complexes and high Mr immune complexes. Based on the twenty-five most abundant proteins, we further suggest that major functions of the larval hemolymph are storage, transport, and immunity. In summary, this study provided first data on constitution, levels, and possible functions of hemolymph proteins in the mosquito larvae, reflecting complex changes occurring in the fight against E. coli infection.
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影响因子：
5.3
作者：
Clayton AM;Dong Y;Dimopoulos G
通讯作者：
Dimopoulos G
影响因子：
3.8
作者：
Cao X;He Y;Hu Y;Zhang X;Wang Y;Zou Z;Chen Y;Blissard GW;Kanost MR;Jiang H
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Jiang H
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Kafatos, FC
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通讯作者：
Jacobs-Lorena, M.