Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children.
Allelic variability of critical virulence genes (eae, bfpA and perA) in typical and atypical enteropathogenic Escherichia coli in Peruvian children.
复制标题
DOI：
10.1099/jmm.0.013706-0
复制
发表时间：
2010-01
影响因子：
3
通讯作者：
Cleary TG
中科院分区：
文献类型：
--
作者：
Contreras CA;Ochoa TJ;Lacher DW;DebRoy C;Navarro A;Talledo M;Donnenberg MS;Ecker L;Gil AI;Lanata CF;Cleary TG
肠致病性大肠杆菌（EPEC）是发展中国家婴儿腹泻的主要原因。本研究的目的是描述关键毒力EPEC基因的等位基因多样性及其与临床特征的关联。应用PCR-限制性片段长度多态性技术对120株EPEC进行了eae（intimin）、bfpA（Ephlin pilin protein of a pilus forming pilus）和perA（plasmid encoded regulator）基因多态性分析。非典型EPEC菌株（eae+，bfp-）是腹泻患儿（54/74，73%）和非腹泻患儿对照组（40/46，87%）中最常见的致病型。总体而言，有13个eae等位基因;最常见的是β（34/120，28%），θ（24/120，20%），kappa（14/120，12%）和mu（8/120，7%）。有5个bfpA等位基因;最常见的是β 1/7（10/26），α 3（7/26）和β 5（3/26）。有3个perA等位基因：β（8/16），α（7/16）和γ（1/16）。菌株属于36个不同的血清群; O 55是最常见的。γ-intimin等位基因在腹泻持续时间较长（>7天）的患者中的频率高于腹泻持续时间较短的患者（3/26，12% vs. 0/48，0%，p<0.05）。与其他等位基因相比，kappa-intimin等位基因具有最高的临床严重程度评分（p<0.05）。在秘鲁儿童中，EPEC菌株的毒力基因高度可变。需要进一步的研究来评估其他毒力标记，以确定特定变异与疾病临床特征之间是否存在关系。
Enteropathogenic Escherichia coli (EPEC) are a leading cause of infantile diarrhea in developing countries. The aim of this study was to describe the allelic diversity of critical virulence EPEC genes and their association with clinical characteristics. 120 EPEC strains isolated from a cohort diarrhea study in Peruvian children were characterized for the allele type of eae (intimin), bfpA (bundlin pilin protein of bundle-forming pilus) and perA (plasmid encoded regulator) genes by PCR-restriction fragment length polymorphism. Atypical EPEC strains (eae+, bfp-) were the most common pathotype in diarrhea (54/74, 73%) and control samples from children without diarrhea (40/46, 87%). Overall there were 13 eae alleles; the most common were beta (34/120, 28%), theta (24/120, 20%), kappa (14/120, 12%) and mu (8/120, 7%). There were 5 bfpA alleles; the most common were beta1/7 (10/26), alpha3 (7/26) and beta5 (3/26). There were 3 perA alleles: beta (8/16), alpha (7/16) and gamma (1/16). The strains belonged to 36 distinct serogroups; O55 was the most frequent. The gamma-intimin allele was more frequently found in diarrhea episodes of longer duration (>7 days) than shorter (3/26, 12% vs. 0/48, 0%, p<0.05). The kappa-intimin allele had the highest clinical severity score in comparison to other alleles (p<0.05). In Peruvian children the virulence genes of EPEC strains are highly variable. Further studies are needed to evaluate additional virulence markers to determine whether relationships exist between specific variants and clinical features of disease.
登录
查看更多内容
影响因子：
6.4
作者：
Hedberg, CW;Savarino, SJ;Tornieporth, N
通讯作者：
Tornieporth, N
影响因子：
3.2
作者：
Lacher, David W.;Steinsland, Hans;Whittam, Thomas S.
通讯作者：
Whittam, Thomas S.
影响因子：
3
作者：
Afset, JE;Bevanger, L;Bergh, K
通讯作者：
Bergh, K
影响因子：
3
作者：
Blanco, Miguel;Blanco, Jesus E.;Blanco, Jorge
通讯作者：
Blanco, Jorge
影响因子：
9.4
作者：
Ito, Kenitiro;Iida, Mariko;Ratchtrachenchai, Orn-Anong
通讯作者：
Ratchtrachenchai, Orn-Anong