General quantitative relations linking cell growth and the cell cycle in Escherichia coli.
General quantitative relations linking cell growth and the cell cycle in Escherichia coli.
复制标题
连接大肠杆菌细胞生长和细胞周期的一般定量关系
DOI：
10.1038/s41564-020-0717-x
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发表时间：
2020-08
影响因子：
28.3
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Zheng H;Bai Y;Jiang M;Tokuyasu TA;Huang X;Zhong F;Wu Y;Fu X;Kleckner N;Hwa T;Liu C
从细胞群体的研究中出现的生长规律为协调细胞生长的全球机制提供了必要的约束。细菌细胞周期研究的基础依赖于50多年前提出的两个相互关联的教条-将细胞质量与生长速率联系起来的Schaechter-Maaloe-Kjeldgaard生长定律和Donachie的生长速率无关的起始质量假说。这些教条激发了许多人努力去理解它们的分子基础和生理后果。虽然他们被普遍接受的快速增长制度，也就是说，倍增时间低于1小时，这些教条的扩展到缓慢增长的制度并没有一贯实现。在这里，通过对大肠杆菌细胞周期在广泛的生长速率范围内的定量生理学研究，我们报告说，无论是在缓慢或快速增长制度的教条。相反，在整个生长速率范围内，发现细胞质量与染色体复制分离速率之间存在线性关系。这些关系使我们提出了一个积分阈值模型，其中的细胞周期是由一个许可证的过程中，其中的速率是相关的染色体动力学在一个简单的方式来控制。这些结果为预测细胞生长-细胞周期关系提供了定量基础。
Growth laws emerging from studies of cell populations provide essential constraints on the global mechanisms that coordinate cell growth. The foundation of bacterial cell cycle studies relies on two interconnected dogmas that were proposed more than 50 years ago—the Schaechter–Maaloe–Kjeldgaard growth law that relates cell mass to growth rate and Donachie’s hypothesis of a growth-rate-independent initiation mass. These dogmas spurred many efforts to understand their molecular bases and physiological consequences. Although they are generally accepted in the fast-growth regime, that is, for doubling times below 1 h, extension of these dogmas to the slow-growth regime has not been consistently achieved. Here, through a quantitative physiological study of Escherichia coli cell cycles over an extensive range of growth rates, we report that neither dogma holds in either the slow- or fast-growth regime. In their stead, linear relations between the cell mass and the rate of chromosome replication–segregation were found across the range of growth rates. These relations led us to propose an integral-threshold model in which the cell cycle is controlled by a licensing process, the rate of which is related in a simple way to chromosomal dynamics. These results provide a quantitative basis for predictive understanding of cell growth–cell cycle relationships.
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影响因子：
2
作者：
BREMER, H;CHURCHWARD, G;YOUNG, R
通讯作者：
YOUNG, R
DOI：
10.1088/1361-6633/aaa628
发表时间：
2018-05
期刊：
Reports on progress in physics. Physical Society (Great Britain)
影响因子：
--
作者：
Jun S;Si F;Pugatch R;Scott M
通讯作者：
Scott M
影响因子：
64.8
作者：
Erickson DW;Schink SJ;Patsalo V;Williamson JR;Gerland U;Hwa T
通讯作者：
Hwa T
DOI：
10.1099/00221287-19-3-592
发表时间：
1958-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
SCHAECHTER, M;MAALOE, O;KJELDGAARD, NO
通讯作者：
KJELDGAARD, NO
影响因子：
5.2
作者：
Kleckner NE;Chatzi K;White MA;Fisher JK;Stouf M
通讯作者：
Stouf M