Superparamagnetic core/shell GoldMag nanoparticles: size-, concentration- and time-dependent cellular nanotoxicity on human umbilical vein endothelial cells and the suitable conditions for magnetic resonance imaging.
Superparamagnetic core/shell GoldMag nanoparticles: size-, concentration- and time-dependent cellular nanotoxicity on human umbilical vein endothelial cells and the suitable conditions for magnetic resonance imaging.
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超顺磁核/壳 GoldMag 纳米粒子：对人脐静脉内皮细胞的尺寸、浓度和时间依赖性细胞纳米毒性以及磁共振成像的合适条件
DOI：
10.1186/s12951-015-0080-x
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发表时间：
2015-03-25
影响因子：
10.2
通讯作者：
Zou L
中科院分区：
文献类型：
--
作者：
Gong M;Yang H;Zhang S;Yang Y;Zhang D;Qi Y;Zou L
背景：金磁纳米颗粒（GMNP）具有胶体金和超顺磁性氧化铁纳米颗粒的性质，使其在药物递送、分离和分子成像等方面具有重要的应用价值。然而，由于纳米效应，GMNP具有高毒性。因此，GMNP的生物安全性应充分研究之前，他们在生物医学中的使用。结果：透射电镜显示GMNP具有典型的壳/核结构，能谱分析证实壳为金结构。30和50 nm的GMNP的平均尺寸分别为30.65 ± 3.15和49.23 ± 5.01 nm，壳层厚度分别为6.8 ± 0.65和8.5 ± 1.36 nm。动态光散射结果表明，30和50 nm GMNP的流体动力学直径分别为33.2 ± 2.68和53.12 ± 4.56 nm。50 nm GMNP的r2弛豫率为98.65 mM（-1）s（-1），而30 nm GMNP的r2弛豫率为80.18 mM（-1）s（-1）。标记HUVECs的增殖、细胞骨架、迁移、微管形成、凋亡和活性氧的产生与GMNP的大小、浓度和作用时间有关。由于较高的标记率，在相同的浓度和时间下，与30 nm GMNP相比，50 nm GMNP表现出显著的纳米毒性增加。结果：50 nm GMNP在25 μg/mL及12 h内对HUVECs无明显的纳米毒性作用，而30 nm GMNP在50 μg/mL及24 h内对HUVECs无明显的纳米毒性作用。更长时间暴露于更大、更高浓度的GMNP会导致HUVEC的标记率和活性氧水平更高。结合r2弛豫率，表明50 nm GMNP更适合于HUVEC标记和MRI，其适宜的浓度和时间为25 μg/mL和12 h。
Background:GoldMag nanoparticles (GMNPs) possess the properties of colloid gold and superparamagnetic iron oxide nanoparticles, which make them useful for delivery, separation and molecular imaging. However, because of the nanometer effect, GMNPs are highly toxic. Thus, the biosafety of GMNPs should be fully studied prior to their use in biomedicine. The main purpose of this study was to evaluate the nanotoxicity of GMNPs on human umbilical vein endothelial cells (HUVECs) and determine a suitable size, concentration and time for magnetic resonance imaging (MRI).Results:Transmission electron microscopy showed that GMNPs had a typical shell/core structure, and the shell was confirmed to be gold using energy dispersive spectrometer analysis. The average sizes of the 30 and 50 nm GMNPs were 30.65 ± 3.15 and 49.23 ± 5.01 nm, respectively, and the shell thickness were 6.8 ± 0.65 and 8.5 ± 1.36 nm, respectively. Dynamic light scattering showed that the hydrodynamic diameter of the 30 and 50 nm GMNPs were 33.2 ± 2.68 and 53.12 ± 4.56 nm, respectively. The r 2 relaxivity of the 50 nm GMNPs was 98.65 mM(-1) s(-1), whereas it was 80.18 mM(-1) s(-1) for the 30 nm GMNPs. The proliferation, cytoskeleton, migration, tube formation, apoptosis and ROS generation of labeled HUVECs depended on the size and concentration of GMNPs and the time of exposure. Because of the higher labeling rate, the 50 nm GMNPs exhibited a significant increase in nanotoxicity compared with the 30 nm GMNPs at the same concentration and time. At no more than 25 μg/mL and 12 hours, the 50 nm GMNPs exhibited no significant nanotoxicity in HUVECs, whereas no toxicity was observed at 50 μg/mL and 24 hours for the 30 nm GMNPs.Conclusions:These results demonstrated that the nanotoxicity of GMNPs in HUVECs depended on size, concentration and time. Exposure to larger GMNPs with a higher concentration for a longer period of time resulted in a higher labeling rate and ROS level for HUVECs. Coupled with r 2 relaxivity, it was suggested that the 50 nm GMNPs are more suitable for HUVEC labeling and MRI, and the suitable concentration and time were 25 μg/mL and 12 hours.
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影响因子：
10.2
作者：
Barathmanikanth S;Kalishwaralal K;Sriram M;Pandian SR;Youn HS;Eom S;Gurunathan S
通讯作者：
Gurunathan S
影响因子：
17.1
作者：
Huang K;Ma H;Liu J;Huo S;Kumar A;Wei T;Zhang X;Jin S;Gan Y;Wang PC;He S;Zhang X;Liang XJ
通讯作者：
Liang XJ
影响因子：
14
作者：
Li, Jasmine J.;Hartono, Deny;Yung, Lin-Yue L.
通讯作者：
Yung, Lin-Yue L.
影响因子：
3.8
作者：
Clift, Martin J. D.;Rothen-Rutishauser, Barbara;Stone, Vicki
通讯作者：
Stone, Vicki
氧化铁纳米颗粒的双重酶样活性及其对减少细胞毒性的意义
影响因子：
17.1
作者：
Chen, Zhongwen;Yin, Jun-Jie;Gu, Ning
通讯作者：
Gu, Ning