Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum-plasma membrane contact sites.
Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulum-plasma membrane contact sites.
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DOI：
10.1093/jxb/erw381
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发表时间：
2016-11
影响因子：
6.9
通讯作者：
Baluska F
中科院分区：
文献类型：
--
作者：
Siao W;Wang P;Voigt B;Hussey PJ;Baluska F
拟南芥synaptotagmin 1定位于与VAP 27 -1不同的ER-PM接触位点，并在维持ER形态和VAP 27 -1的动力学方面发挥作用。拟南芥突触结合蛋白1（SYT 1）定位于叶和根细胞的内质网-质膜（ER-PM）接触位点。拟南芥SYT 1的ER-PM定位类似于动物细胞中的扩展突触结合蛋白（E-SYTs）。在哺乳动物中，E-SYT已被证明可以调节钙信号传导、脂质转移和内吞作用。据报道，拟南芥SYT 1是维持细胞完整性和病毒运动所必需的。这项研究提供了详细的了解SYT 1和VAP 27 -1，另一个ER-PM-tethering蛋白的亚细胞定位。SYT 1和VAP 27 -1被证明定位于不同的ER-PM接触位点。VAP 27 -1富集的ER-PM接触位点（V-EPCS）始终与SYT 1富集的ER-PM接触位点（S-EPCS）接触。SYT 1无效突变体的V-EPCSs仍然存在于叶表皮细胞中，但它们的稳定性低于野生型。SYT 1无效突变体的叶细胞中，皮层ER的多边形网络解体，VAP 27 -1蛋白在ER-PM接触位点上的移动性增加。这些结果表明SYT 1负责稳定ER网络和V-EPCSs。
Arabidopsis synaptotagmin 1 is localized on ER–PM contact sites distinct from VAP27-1 and plays roles in maintaining ER morphology and the dynamics of VAP27-1. Arabidopsis synaptotagmin 1 (SYT1) is localized on the endoplasmic reticulum–plasma membrane (ER–PM) contact sites in leaf and root cells. The ER–PM localization of Arabidopsis SYT1 resembles that of the extended synaptotagmins (E-SYTs) in animal cells. In mammals, E-SYTs have been shown to regulate calcium signaling, lipid transfer, and endocytosis. Arabidopsis SYT1 was reported to be essential for maintaining cell integrity and virus movement. This study provides detailed insight into the subcellular localization of SYT1 and VAP27-1, another ER–PM-tethering protein. SYT1 and VAP27-1 were shown to be localized on distinct ER–PM contact sites. The VAP27-1-enriched ER–PM contact sites (V-EPCSs) were always in contact with the SYT1-enriched ER–PM contact sites (S-EPCSs). The V-EPCSs still existed in the leaf epidermal cells of the SYT1 null mutant; however, they were less stable than those in the wild type. The polygonal networks of cortical ER disassembled and the mobility of VAP27-1 protein on the ER–PM contact sites increased in leaf cells of the SYT1 null mutant. These results suggest that SYT1 is responsible for stabilizing the ER network and V-EPCSs.
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DOI：
10.1083/jcb.13.3.405
发表时间：
1962-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
ROSENBLUTH J
通讯作者：
ROSENBLUTH J
影响因子：
64.5
作者：
Giordano F;Saheki Y;Idevall-Hagren O;Colombo SF;Pirruccello M;Milosevic I;Gracheva EO;Bagriantsev SN;Borgese N;De Camilli P
通讯作者：
De Camilli P
DOI：
10.1073/pnas.1306331110
发表时间：
2013-07-16
影响因子：
11.1
作者：
Mehrshahi, Payam;Stefano, Giovanni;DellaPenna, Dean
通讯作者：
DellaPenna, Dean
影响因子：
5.2
作者：
Moghadam PK;Jackson MB
通讯作者：
Jackson MB
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF