Measurement of glycine in the human brain in vivo by 1H-MRS at 3 T: application in brain tumors.
Measurement of glycine in the human brain in vivo by 1H-MRS at 3 T: application in brain tumors.
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DOI：
10.1002/mrm.22857
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发表时间：
2011-09
影响因子：
3.3
通讯作者：
Maher, Elizabeth A.
中科院分区：
文献类型：
--
作者：
Choi, Changho;Ganji, Sandeep K.;DeBerardinis, Ralph J.;Dimitrov, Ivan E.;Pascual, Juan M.;Bachoo, Robert;Mickey, Bruce E.;Malloy, Craig R.;Maher, Elizabeth A.
关键词：
甘氨酸（Gly）是合成蛋白质、核酸和其他分子所需的关键代谢中间体，因此在癌症中检测它可以提供有关肿瘤生长的生物学相关信息。在这里，我们报告了通过优化的点分辨光谱（PRESS）序列在3T下测量人脑和胶质瘤中的Gly。采用三维体积定位的数值模拟方法研究了主要障碍物肌醇（mI）的回声时间依赖性。模拟结果表明，一个亚becho对(TE1, TE2) = (60,100) ms可以同时检测到Gly和mI，并具有最佳的选择性。在5名健康志愿者的右侧顶叶皮层上对优化后的PRESS进行了体内验证。根据LCModel估计的代谢物信号相对于脑水信号进行归一化，并假设总肌酸浓度为8 mM，评估浓度。Gly浓度估计为0.6±0.1 mM（平均值±SD， n=5），平均cram<s:2> - rao下界为9±1%。应用PRESS序列测定多形性胶质母细胞瘤患者的Gly水平。利用肿瘤肿块的水信号获得代谢物浓度。研究显示，人类神经胶质瘤的一个亚群中甘氨酸的含量比正常人高1.5-8倍。
Glycine (Gly) is a key metabolic intermediate required for the synthesis of proteins, nucleic acids and other molecules, and its detection in cancer could therefore provide biologically relevant information about the growth of the tumor. Here we report measurement of Gly in human brain and gliomas by an optimized point-resolved spectroscopy (PRESS) sequence at 3T. Echo time dependence of the major obstacle, myo-inositol (mI) multiplet, was investigated with numerical simulations, incorporating the 3D volume localization. The simulations indicated that a subecho pair (TE1, TE2) = (60, 100) ms permits detection of both Gly and mI with optimum selectivity. In vivo validation of the optimized PRESS was conducted on the right parietal cortex of five healthy volunteers. Metabolite signals estimated from LCModel were normalized with respect to the brain water signal and the concentrations were evaluated assuming the total creatine concentration at 8 mM. The Gly concentration was estimated as 0.6±0.1 mM (mean±SD, n=5), with a mean Cramér-Rao lower bound of 9±1%. The PRESS sequence was applied to measure the Gly levels in patients with glioblastoma multiforme. Metabolite concentrations were obtained using the water signal from the tumor mass. The study revealed that a subset of human gliomas contains glycine levels elevated 1.5–8 fold relative to normal.
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影响因子：
5.9
作者：
Huisman, TAGM;Thiel, T;Martin, E
通讯作者：
Martin, E
影响因子：
2.9
作者：
KOTITSCHKE, K;JUNG, H;BOGDAHN, U
通讯作者：
BOGDAHN, U
影响因子：
3.3
作者：
Marjanska, Malgorzata;Henry, Pierre-Gilles;Gruetter, Rolf
通讯作者：
Gruetter, Rolf
DOI：
10.1007/s10334-008-0145-z
发表时间：
2009-02-01
影响因子：
2.3
作者：
Hattingen, Elke;Lanfermann, Heinrich;Pilatus, Ulrich
通讯作者：
Pilatus, Ulrich
影响因子：
3.3
作者：
GRUETTER, R
通讯作者：
GRUETTER, R