A Folding-Based Electrochemical Aptasensor for the Single-Step Detection of the SARS-CoV-2 Spike Protein.
A Folding-Based Electrochemical Aptasensor for the Single-Step Detection of the SARS-CoV-2 Spike Protein.
复制标题
折叠式电化学适配传感器用于SARS-CoV-2刺突蛋白的一步检测
DOI：
10.1021/acsami.2c02405
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发表时间：
2022-05-04
影响因子：
9.5
通讯作者：
Careri M
中科院分区：
文献类型：
--
作者：
Curti F;Fortunati S;Knoll W;Giannetto M;Corradini R;Bertucci A;Careri M
高效及时的检测已成为当前COVID-19疫情管理、控制和监测的中心环节。需要简单，快速，具有成本效益的诊断，可以补充目前的聚合酶链反应为基础的方法和侧流免疫测定。在这里，我们报告的电化学传感平台的基础上单壁碳纳米管丝网印刷电极（SWCNT-SPE）功能化与氧化还原标记的DNA适体，特异性结合到SARS-CoV-2刺突蛋白S1亚基的受体结合域的发展。S1蛋白的单步无试剂检测是通过DNA适体的结合诱导的浓度依赖性折叠来实现的，所述DNA适体的结合诱导的浓度依赖性折叠降低了氧化还原标签和电极表面之间的电子转移过程的效率，并导致所得安培信号的抑制。该适体传感器对靶S1蛋白具有特异性，解离常数（KD）值为43 ± 4 nM，检测限为7 nM。我们证明，目标S1蛋白可以在缓冲液和广泛用于收集鼻咽拭子的人工病毒转运介质中检测到，并且在不同的非目标病毒蛋白存在下没有观察到交叉反应性。我们预计，这种基于SWCNT-SPE的电化学适体传感器形式将证明可用于检测核酸适体配体可用于的其他蛋白质靶。
Efficient and timely testing has taken center stage in the management, control, and monitoring of the current COVID-19 pandemic. Simple, rapid, cost-effective diagnostics are needed that can complement current polymerase chain reaction-based methods and lateral flow immunoassays. Here, we report the development of an electrochemical sensing platform based on single-walled carbon nanotube screen-printed electrodes (SWCNT-SPEs) functionalized with a redox-tagged DNA aptamer that specifically binds to the receptor binding domain of the SARS-CoV-2 spike protein S1 subunit. Single-step, reagentless detection of the S1 protein is achieved through a binding-induced, concentration-dependent folding of the DNA aptamer that reduces the efficiency of the electron transfer process between the redox tag and the electrode surface and causes a suppression of the resulting amperometric signal. This aptasensor is specific for the target S1 protein with a dissociation constant (KD) value of 43 ± 4 nM and a limit of detection of 7 nM. We demonstrate that the target S1 protein can be detected both in a buffer solution and in an artificial viral transport medium widely used for the collection of nasopharyngeal swabs, and that no cross-reactivity is observed in the presence of different, non-target viral proteins. We expect that this SWCNT-SPE-based format of electrochemical aptasensor will prove useful for the detection of other protein targets for which nucleic acid aptamer ligands are made available.
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DOI：
10.1016/j.snb.2021.130377
发表时间：
2021-10-15
期刊：
Sensors and actuators. B, Chemical
影响因子：
--
作者：
Amouzadeh Tabrizi M;Nazari L;Acedo P
通讯作者：
Acedo P
影响因子：
16.6
作者：
Bertucci, Alessandro;Porchetta, Alessandro;Ricci, Francesco
通讯作者：
Ricci, Francesco
用于快速检测 SARS-CoV-2 和甲型 H1N1 流感病毒的多通道免疫传感器平台
影响因子：
9.5
作者：
Li, Jianyong;Lin, Rui;Hao, Rongzhang
通讯作者：
Hao, Rongzhang
影响因子：
7.4
作者：
Arroyo-Curras, Netzahualcoyotl;Scida, Karen;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
DOI：
10.1002/anie.202107730
发表时间：
2021-09-20
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Kacherovsky N;Yang LF;Dang HV;Cheng EL;Cardle II;Walls AC;McCallum M;Sellers DL;DiMaio F;Salipante SJ;Corti D;Veesler D;Pun SH
通讯作者：
Pun SH