The mating type locus protein MAT1-2-1 of Trichoderma reesei interacts with Xyr1 and regulates cellulase gene expression in response to light.
The mating type locus protein MAT1-2-1 of Trichoderma reesei interacts with Xyr1 and regulates cellulase gene expression in response to light.
复制标题
里氏木霉交配型基因座蛋白 MAT1-2-1 与 Xyr1 相互作用并响应光调节纤维素酶基因表达
DOI：
10.1038/s41598-017-17439-2
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发表时间：
2017-12-11
影响因子：
4.6
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Zheng F;Cao Y;Wang L;Lv X;Meng X;Zhang W;Chen G;Liu W
纤维素分解真菌里氏木霉（Trichoderma reesei）的纤维素酶生产受到多种环境和生理条件的影响，这些条件涉及具有多个转录因子的复杂调控网络。在这里，我们确定了交配型基因座蛋白MAT 1 -2-1作为T. reesei纤维素酶基因。酵母双杂交和GST pulldown分析表明，MAT 1 -2-1与Xyr 1的转录激活区（AD，767~940 aa）和中间同源区（MHR 2，314~632 aa）直接相互作用。mat 1 -2-1基因的破坏损害了纤维素酶基因的诱导表达与Avicel响应于光或乳糖。染色质免疫沉淀（ChIP）表明，MAT 1 -2-1招募到cbh 1（纤维二糖水解酶1编码）基因启动子中的Xyr 1依赖性的方式。这些结果强烈支持MAT 1 -2-1作为Xyr 1生理辅因子的重要作用，并表明MAT 1 -2-1代表另一个调节节点，该节点将光响应与碳源信号传导整合起来，以微调纤维素酶基因转录。
Cellulase production in the model cellulolytic fungus Trichoderma reesei is subject to a variety of environmental and physiological conditions involving an intricate regulatory network with multiple transcription factors. Here, we identified the mating type locus protein MAT1-2-1 as an interacting partner for the key transcriptional activator Xyr1 of T. reesei cellulase genes. Yeast two-hybrid and GST pulldown analyses revealed that MAT1-2-1 directly interacted with the putative transcription activation domain (AD, 767~940 aa) and the middle homology region (MHR2, 314~632 aa) of Xyr1. Disruption of the mat1-2-1 gene compromised the induced expression of cellulase genes with Avicel in response to light or with lactose. Chromatin immunoprecipitation (ChIP) demonstrated that MAT1-2-1 was recruited to the cbh1 (cellobiohydrolase 1-encoding) gene promoter in a Xyr1-dependent manner. These results strongly support an important role of MAT1-2-1 as a physiological cofactor of Xyr1, and suggest that MAT1-2-1 represents another regulatory node that integrates the light response with carbon source signaling to fine tune cellulase gene transcription.
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影响因子：
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通讯作者：
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通讯作者：
Pakula TM