An open source, wireless capable miniature microscope system.
An open source, wireless capable miniature microscope system.
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DOI：
10.1088/1741-2552/aa6806
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发表时间：
2017-08
影响因子：
4
通讯作者：
Gardner TJ
中科院分区：
文献类型：
--
作者：
Liberti WA;Perkins LN;Leman DP;Gardner TJ
通过头戴式显微镜对自由活动的动物进行荧光成像正在成为研究神经回路功能的标准方法。需要灵活的开源设计来促进该方法的发展。我们描述了一种用于对自由行为的动物进行单光子荧光成像的微型显微镜。该设备由 3D 打印零件和现成组件制成。这些显微镜重量不到 1.8 克，可配置为对各种荧光团进行成像，并且可以无线使用或与有源换向器结合使用。基于适用于 macOS 的 Swift 的显微镜控制软件为闭环或 BMI 实验提供低延迟图像处理功能。微型显微镜被部署在鸣禽斑胸草雀的运动前区 HVC（用作专有名称）。单个神经元产生时间上精确的钙活动模式，这些模式在歌曲的重复演绎中保持一致。在数周和数月的时间尺度内对多个细胞进行了追踪，为研究 HVC 中与学习相关的变化提供了机会。 3D 打印微型显微镜完全由消费级组件组成，是头戴式成像的一种经济高效的模块化选择。这些易于构建和可定制的工具可以在数周的时间范围内访问细胞类型特定的神经集合。
Fluorescence imaging through head-mounted microscopes in freely behaving animals is becoming a standard method to study neural circuit function. Flexible, open-source designs are needed to spur evolution of the method. We describe a miniature microscope for single-photon fluorescence imaging in freely behaving animals. The device is made from 3D printed parts and off-the-shelf components. These microscopes weigh less than 1.8 g, can be configured to image a variety of fluorophores, and can be used wirelessly or in conjunction with active commutators. Microscope control software, based in Swift for macOS, provides low-latency image processing capabilities for closed-loop, or BMI, experiments. Miniature microscopes were deployed in the songbird premotor region HVC (used as a proper name), in singing zebra finches. Individual neurons yield temporally precise patterns of calcium activity that are consistent over repeated renditions of song. Several cells were tracked over timescales of weeks and months, providing an opportunity to study learning related changes in HVC. 3D printed miniature microscopes, composed completely of consumer grade components, are a cost-effective, modular option for head-mounting imaging. These easily constructed and customizable tools provide access to cell-type specific neural ensembles over timescales of weeks.
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影响因子：
9.8
作者：
Markowitz JE;Liberti WA 3rd;Guitchounts G;Velho T;Lois C;Gardner TJ
通讯作者：
Gardner TJ
影响因子：
7.7
作者：
Rubin, Alon;Geva, Nitzan;Ziv, Yaniv
通讯作者：
Ziv, Yaniv
影响因子：
14.8
作者：
Resendez SL;Jennings JH;Ung RL;Namboodiri VM;Zhou ZC;Otis JM;Nomura H;McHenry JA;Kosyk O;Stuber GD
通讯作者：
Stuber GD
影响因子：
25
作者：
Dombeck, Daniel A.;Harvey, Christopher D.;Tian, Lin;Looger, Loren L.;Tank, David W.
通讯作者：
Tank, David W.
影响因子：
4
作者：
Guitchounts G;Markowitz JE;Liberti WA;Gardner TJ
通讯作者：
Gardner TJ