Age-dependent remodeling of gut microbiome and host serum metabolome in mice.
Age-dependent remodeling of gut microbiome and host serum metabolome in mice.
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DOI：
10.18632/aging.202525
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发表时间：
2021-02-17
期刊：
影响因子：
--
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Wu CS;Muthyala SDV;Klemashevich C;Ufondu AU;Menon R;Chen Z;Devaraj S;Jayaraman A;Sun Y
微生物群和宿主代谢之间的相互作用在健康中起着重要作用。在这里，我们研究了雄性C57 BL/6J小鼠的年龄，肠道微生物组和宿主血清代谢物之间的关系。对3、6、18和28个月龄（M）小鼠的粪便微生物组分析显示，厚壁菌门/拟杆菌门比率在6个月龄组中最高;厚壁菌门在老年组中的减少表明肠道微生物群从食物中收获能量的能力降低。我们发现变形菌的年龄依赖性增加，这可能导致粘液结构改变，更容易被细菌渗透，最终增加肠道炎症。3、12、18和28 M小鼠在进食状态下的极性血清代谢物的代谢组学分析显示，色氨酸、嘌呤、氨基酸和烟酰胺代谢涉及的代谢级联发生了年龄相关变化。相关分析表明烟酰胺随年龄增长而下降，而嘌呤代谢产物尿囊素和鸟苷随年龄增长而增加。值得注意的是，色氨酸及其微生物衍生的化合物吲哚和吲哚-3-乳酸随年龄显著降低，而犬尿氨酸随年龄增加。总之，这些结果表明细菌和宿主代谢之间存在显著的相互作用，肠道生态失调和微生物代谢的改变有助于衰老。
The interplay between microbiota and host metabolism plays an important role in health. Here, we examined the relationship between age, gut microbiome and host serum metabolites in male C57BL/6J mice. Fecal microbiome analysis of 3, 6, 18, and 28 months (M) old mice showed that the Firmicutes/Bacteroidetes ratio was highest in the 6M group; the decrease of Firmicutes in the older age groups suggests a reduced capacity of gut microflora to harvest energy from food. We found age-dependent increase in Proteobacteria, which may lead to altered mucus structure more susceptible to bacteria penetration and ultimately increased intestinal inflammation. Metabolomic profiling of polar serum metabolites at fed state in 3, 12, 18 and 28M mice revealed age-associated changes in metabolic cascades involved in tryptophan, purine, amino acids, and nicotinamide metabolism. Correlation analyses showed that nicotinamide decreased with age, while allantoin and guanosine, metabolites in purine metabolism, increased with age. Notably, tryptophan and its microbially derived compounds indole and indole-3-lactic acid significantly decreased with age, while kynurenine increased with age. Together, these results suggest a significant interplay between bacterial and host metabolism, and gut dysbiosis and altered microbial metabolism contribute to aging.
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影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
影响因子：
9.3
作者：
Devaraj S;Hemarajata P;Versalovic J
通讯作者：
Versalovic J
影响因子：
16.6
作者：
Cheng, Susan;Larson, Martin G.;McCabe, Elizabeth L.;Murabito, Joanne M.;Rhee, Eugene P.;Ho, Jennifer E.;Jacques, Paul F.;Ghorbani, Anahita;Magnusson, Martin;Souza, Amanda L.;Deik, Amy A.;Pierce, Kerry A.;Bullock, Kevin;O'Donnell, Christopher J.;Melander, Olle;Clish, Clary B.;Vasan, Ramachandran S.;Gerszten, Robert E.;Wang, Thomas J.
通讯作者：
Wang, Thomas J.
影响因子：
64.8
作者：
Claesson, Marcus J.;Jeffery, Ian B.;O'Toole, Paul W.
通讯作者：
O'Toole, Paul W.
DOI：
10.18632/aging.100961
发表时间：
2016-05
期刊：
Aging
影响因子：
--
作者：
Ivanisevic J;Stauch KL;Petrascheck M;Benton HP;Epstein AA;Fang M;Gorantla S;Tran M;Hoang L;Kurczy ME;Boska MD;Gendelman HE;Fox HS;Siuzdak G
通讯作者：
Siuzdak G