Topically Applied Biopolymer-Based Tri-Layered Hierarchically Structured Nanofibrous Scaffold with a Self-Pumping Effect for Accelerated Full-Thickness Wound Healing in a Rat Model.
Topically Applied Biopolymer-Based Tri-Layered Hierarchically Structured Nanofibrous Scaffold with a Self-Pumping Effect for Accelerated Full-Thickness Wound Healing in a Rat Model.
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DOI：
10.3390/pharmaceutics15051518
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发表时间：
2023-05-17
期刊：
影响因子：
5.4
通讯作者：
Soliman HMA
中科院分区：
文献类型：
--
作者：
Hamza KH;El-Shanshory AA;Agwa MM;Abo-Alkasem MI;El-Fakharany EM;Abdelsattar AS;El-Bardan AA;Kassem TS;Mo X;Soliman HMA
伤口愈合已成为全球范围内的重大问题。大多数基于伤口敷料的生物聚合物缺乏多功能性，使其无法满足所有临床要求。因此，伤口敷料中的基于多功能生物聚合物的三层分级纳米纤维支架可以有助于皮肤再生。在这项研究中，一个多功能的抗菌生物聚合物为基础的三层分级纳米纤维支架，包括三层构建。底层和顶层分别含有亲水性丝素蛋白（SF）和鱼皮胶原蛋白（COL），用于加速愈合，中间穿插含有阿莫西林（AMX）作为抗菌药物的疏水性聚-3-羟基丁酸酯（PHB）。通过SEM、FTIR、流体吸收、接触角、孔隙率和机械性能来估计纳米纤维支架的有利的物理化学性质。MTT法和细胞划痕法分别检测了材料的细胞毒性和细胞愈合能力，结果表明材料具有良好的生物相容性。纳米纤维支架对多种病原菌表现出显着的抗微生物活性。此外，体内伤口愈合和组织学研究表明，创伤大鼠在第14天伤口完全愈合，伴随着转化生长因子-β 1（TGF-β 1）表达水平的增加和白细胞介素-6（IL-6）表达水平的降低，沿着。结果表明，所制造的纳米纤维支架是一种有效的伤口敷料支架，并在大鼠模型中显著加速全层伤口愈合。
Wound healing has grown to be a significant problem at a global scale. The lack of multifunctionality in most wound dressing-based biopolymers prevents them from meeting all clinical requirements. Therefore, a multifunctional biopolymer-based tri-layered hierarchically nanofibrous scaffold in wound dressing can contribute to skin regeneration. In this study, a multifunctional antibacterial biopolymer-based tri-layered hierarchically nanofibrous scaffold comprising three layers was constructed. The bottom and the top layers contain hydrophilic silk fibroin (SF) and fish skin collagen (COL), respectively, for accelerated healing, interspersed with a middle layer of hydrophobic poly-3-hydroxybutyrate (PHB) containing amoxicillin (AMX) as an antibacterial drug. The advantageous physicochemical properties of the nanofibrous scaffold were estimated by SEM, FTIR, fluid uptake, contact angle, porosity, and mechanical properties. Moreover, the in vitro cytotoxicity and cell healing were assessed by MTT assay and the cell scratching method, respectively, and revealed excellent biocompatibility. The nanofibrous scaffold exhibited significant antimicrobial activity against multiple pathogenic bacteria. Furthermore, the in vivo wound healing and histological studies demonstrated complete wound healing in wounded rats on day 14, along with an increase in the expression level of the transforming growth factor-β1 (TGF-β1) and a decrease in the expression level of interleukin-6 (IL-6). The results revealed that the fabricated nanofibrous scaffold is a potent wound dressing scaffold, and significantly accelerates full-thickness wound healing in a rat model.
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来自替代性廉价碳源上种植的不同细菌菌株的poly-ß-羟基丁酸（PHB）的生物合成。
影响因子：
5
作者：
El-Kadi SM;Elbagory M;El-Zawawy HAH;El-Shaer HFA;Shoukry AA;El-Nahrawy S;Omara AE;Ali DFI
通讯作者：
Ali DFI
影响因子：
2.6
作者：
Elbialy ZI;Atiba A;Abdelnaby A;Al-Hawary II;Elsheshtawy A;El-Serehy HA;Abdel-Daim MM;Fadl SE;Assar DH
通讯作者：
Assar DH
DOI：
10.1016/j.ijbiomac.2018.05.099
发表时间：
2018-09-01
影响因子：
8.2
作者：
Chanda, Amit;Adhikari, Jaideep;Saha, Prosenjit
通讯作者：
Saha, Prosenjit
影响因子：
3.8
作者：
Bebu, Andreea;Szabo, Laszlo;David, Leontin
通讯作者：
David, Leontin
影响因子：
5.8
作者：
Agwa, Mona M.;Abu-Serie, Marwa M.;Sabra, Sally
通讯作者：
Sabra, Sally