Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression.
Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression.
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DOI：
10.1186/s12917-020-02566-2
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发表时间：
2020-09-24
影响因子：
2.6
通讯作者：
Assar DH
中科院分区：
文献类型：
--
作者：
Elbialy ZI;Atiba A;Abdelnaby A;Al-Hawary II;Elsheshtawy A;El-Serehy HA;Abdel-Daim MM;Fadl SE;Assar DH
胶原蛋白是哺乳动物结缔组织中最丰富的结构蛋白，约占动物蛋白的 30%。目前的研究评估了尼罗罗非鱼皮肤胶原蛋白提取物改善大鼠皮肤伤口愈合的潜在能力，并研究了潜在的可能机制。使用大鼠模型，实验设计包括对照组（CG）和罗非鱼胶原治疗组（TCG）。全身麻醉下对所有大鼠背部进行全层创面，然后将罗非鱼胶原提取物局部涂抹于TCG创面部位。在受伤后第0、3、6、9、12和15天测量两个实验组的伤口面积。组织病理学检测创面肉芽组织分期，免疫组化检测血管内皮生长因子（VEGF）、转化生长因子（TGF-β1）的表达。此外，通过实时 qPCR 对转化生长因子-β (TGF-ß1)、碱性成纤维细胞生长因子 (bFGF) 和 α-平滑肌肌动蛋白 (α-SMA) 的相对基因表达分析进行定量。组织病理学评估显示，与 CG 相比，TCG 具有明显的皮肤愈合迹象。免疫组织化学结果显示TCG中VEGF和TGF-β1的表达水平显着增强。此外，TCG 表现出 VEGF、bFGF 和 α-SMA 基因表达的显着上调。这些发现表明，局部应用尼罗罗非鱼胶原提取物可以促进大鼠皮肤伤口愈合过程，这可能归因于其对招募和激活巨噬细胞产生趋化生长因子、成纤维细胞增殖和血管生成的刺激作用。因此，胶原蛋白提取物可能成为皮肤伤口愈合治疗的潜在生物材料。
Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. Moreover, relative gene expression analysis of transforming growth factor-beta (TGF-ß1), basic fibroblast growth factor (bFGF), and alpha-smooth muscle actin (α-SMA) were quantified by real-time qPCR. The histopathological assessment showed noticeable signs of skin healing in TCG compared to CG. Immunohistochemistry results revealed remarkable enhancement in the expression levels of VEGF and TGF-β1 in TCG. Furthermore, TCG exhibited marked upregulation in the VEGF, bFGF, and α-SMA genes expression. These findings suggested that the topical application of Nile tilapia collagen extract can promote the cutaneous wound healing process in rats, which could be attributed to its stimulating effect on recruiting and activating macrophages to produce chemotactic growth factors, fibroblast proliferation, and angiogenesis. The collagen extract could, therefore, be a potential biomaterial for cutaneous wound healing therapeutics.
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DOI：
10.2147/ccid.s50046
发表时间：
2014
期刊：
Clinical, cosmetic and investigational dermatology
影响因子：
--
作者：
Darby IA;Laverdet B;Bonté F;Desmoulière A
通讯作者：
Desmoulière A
影响因子：
1.2
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通讯作者：
Uzuka, Yuji
DOI：
10.1016/j.bbrc.2016.03.121
发表时间：
2016-04-29
影响因子：
3.1
作者：
Ennaas, Nadia;Hammami, Riadh;Fliss, Ismail
通讯作者：
Fliss, Ismail
DOI：
10.1152/ajpendo.00019.2003
发表时间：
2003-12-01
影响因子：
5.1
作者：
Nakahara, T;Hashimoto, K;Preedy, VR
通讯作者：
Preedy, VR
DOI：
10.1016/j.bbrc.2019.04.198
发表时间：
2019-06-30
影响因子：
3.1
作者：
Li, Ding;Yuan, Qi;Li, Aoyu
通讯作者：
Li, Aoyu