Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources.
Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources.
复制标题
来自替代性廉价碳源上种植的不同细菌菌株的poly-ß-羟基丁酸（PHB）的生物合成。
DOI：
10.3390/polym13213801
复制
发表时间：
2021-11-03
期刊：
影响因子：
5
通讯作者：
Ali DFI
中科院分区：
文献类型：
--
作者：
El-Kadi SM;Elbagory M;El-Zawawy HAH;El-Shaer HFA;Shoukry AA;El-Nahrawy S;Omara AE;Ali DFI
在三种不同的培养基上测试了 30 种细菌分离株的聚-β-羟基丁酸酯 (PHB) 生产。基于分子生物学筛选鉴定出生产PHB的最佳菌株；然后，使用三种不同的替代碳源（乳清粉、甜菜糖蜜和枣糖蜜），通过红外（IR）光谱和气相色谱-质谱（GC-MS/MS）分析评估物理性质。我们的结果表明，基于分子生物学鉴定的最佳分离株是副丝状芽孢杆菌 MCCC 1A04098、盐固氮菌 NBRC 102611 和内姜山短波单胞菌 BIO-TAS2-2。在A. salinestris培养基中添加甜菜糖蜜增加了细胞干重（CDW）、PHB浓度、PHB%和转化系数（分别为4.97 g/L、1.56 g/L、31.38%和23.92%）。 PHB g/L 和 CDW g/L 之间的相关系数值在非常强和中等正相关之间变化。副蕈状芽孢杆菌和盐芽孢杆菌产生的 PHB 的 IR 显示出相似的条带，证实了 PHB 的存在；然而，B. naejangsanensis 显示出较弱的条带，表明 PHB 浓度较低。获得的化学成分表明，提取的PHB的GC-MS对于副丝状芽孢杆菌来说代表2,4-二叔丁基苯酚，对于A. salinestris和Brevundimonas naejangsanensis两者来说代表2-丁烯酸的异丙酯。因此，微生物产生的PHB可以被认为是一种可生物降解的聚酯，并且代表了开发环保和完全可生物降解塑料的一种有前景的技术。
Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were Bacillus paramycoides MCCC 1A04098, Azotobacter salinestris NBRC 102611 and Brevundimonas naejangsanensis BIO-TAS2-2. The addition of sugar beet molasses to the medium of A. salinestris increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the produced PHB from B. paramycoides and A. salinestris showed similar bands which confirmed the presence of PHB; however, B. naejangsanensis showed weak bands, indicating lower PHB concentration. The chemical composition obtained showed that the GC-MS of the PHB extracted represents 2, 4-ditert-butylphenol for B. paramycoides and isopropyl ester of 2-butenoic acid for both of A. salinestris and Brevundimonas naejangsanensis. Therefore, PHB produced by microorganisms can be considered a biodegradable polyester, and represents a promising technique for the development of eco-friendly and fully biodegradable plastics.
登录
查看更多内容
影响因子：
6.7
作者：
Gouda, MK;Swellam, AE;Omar, SH
通讯作者：
Omar, SH
DOI：
10.1099/00221287-110-2-393
发表时间：
1979-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
CARTER, IS;DAWES, EA
通讯作者：
DAWES, EA
影响因子：
4.1
作者：
Carlozzi, Pietro;Touloupakis, Eleftherios;Lazzeri, Andrea
通讯作者：
Lazzeri, Andrea
DOI：
10.1111/j.1365-2672.1968.tb00367.x
发表时间：
1968-01-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
ABDELMAL.Y;ISHAC, YZ
通讯作者：
ISHAC, YZ
影响因子：
4
作者：
Borah, B;Thakur, PS;Nigam, JN
通讯作者：
Nigam, JN