Analysis of postnatal eye development in the mouse with high-resolution small animal magnetic resonance imaging.
Analysis of postnatal eye development in the mouse with high-resolution small animal magnetic resonance imaging.
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DOI：
10.1167/iovs.08-2767
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发表时间：
2010-01
影响因子：
4.4
通讯作者：
Tkatchenko AV
中科院分区：
文献类型：
--
作者：
Tkatchenko TV;Shen Y;Tkatchenko AV
小鼠近视的研究由于难以准确测量在活体生长的小鼠眼睛中观察到的微小变化，以及缺乏关于屈光发育的数据而变得复杂。本研究的目的是对小鼠眼睛的生长和屈光发育进行体内高分辨率分析。用高分辨率小动物MRI和高分辨率红外验光仪分析了P21-p89C57BL/6J小鼠的屈光发育。小鼠眼球生长在P40后减速。在生长过程中，眼睛保持略微拉长的形状。前房生长模式相似，角膜曲率半径(CRC)呈线性增加。晶状体的生长速度在89岁之前一直保持不变。晶状体在P40“过度生长”，导致玻璃体腔深度(VCD)下降。小鼠出现近视屈光不正的年龄较小(−为13.2±2.0D，Mean±SD，P21)。屈光不正在P32左右稳定在正视值附近，并一直保持正视，直到P40。随着年龄的增长，小鼠逐渐变得远视(+1.2±1.7D，P67；+3.6±2.3D，P89)。小鼠的眼睛成分发育类似于树鼠，但与高等灵长类动物和人类略有不同。主要差异可归因于晶体晶状体和CRC的年龄相关变化。尽管有这些差异，小鼠似乎能够在P32-P40达到并保持正视屈光状态。
Studies of myopia in mice have been complicated by the difficulty in obtaining accurate measurements of small changes observed in the growing mouse eye in vivo and the lack of data on refractive eye development. The purpose of this study was to carry out an in vivo high-resolution analysis of mouse eye growth and refractive development. A high-resolution small animal MRI and a high-resolution infrared photorefractor were used to analyze refractive development in P21-P89 C57BL/6J mice. The growth of the mouse eye decelerated after P40. The eye maintained a slightly prolate shape during growth. The anterior chamber growth exhibited similar pattern, while corneal radius of curvature (CRC) increased linearly. The growth rate of the lens remained constant until P89. The lens “overgrew” the eye at P40 resulting in a decline in vitreous chamber depth (VCD). Mice showed myopic refractive errors at younger age (−13.2 ± 2.0 D, mean ± SD, P21). The refractive errors stabilized around emmetropic values by P32 and remained emmetropic until P40. Mice became progressively hyperopic with age (+1.2 ± 1.7 D, P67; +3.6 ± 2.3 D, P89). Development of ocular components in the mouse is similar to the tree shrew, but somewhat different from higher primates and humans. Main differences can be attributed to the age-related changes of the crystalline lens and CRC. In spite of these differences, mice appear to be able to achieve and maintain emmetropic refractive status at P32-P40.
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影响因子：
2.8
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