Multiple functional sites on a single Ia molecule defined using T cell clones and antibodies with chain-determined specificity.
Multiple functional sites on a single Ia molecule defined using T cell clones and antibodies with chain-determined specificity.
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DOI：
10.1084/jem.159.3.704
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发表时间：
1984-03-01
期刊：
影响因子：
--
通讯作者：
Fathman CG
中科院分区：
文献类型：
--
作者：
Frelinger JG;Shigeta M;Infante AJ;Nelson PA;Pierres M;Fathman CG
使用单克隆抗体（mAb）抑制抗原反应性（C57 BL 6/J X A/J）F1限制性T细胞克隆的增殖。我们已经能够将这些F1限制性T细胞克隆细分为两组：一组识别A α k A β B分子，另一组识别A α B A β k分子。使用具有确定反应性的克隆，我们可以将单克隆抗体的反应性分配给A α或A β链。通过免疫沉淀和二维分析Ia分子从F1脾细胞，我们可以独立映射的单克隆抗体的反应性被确定的A α或A β链。迄今为止，这两种抗体反应性的链定位方法已经达成一致。此外，A α k A β B限制性T细胞克隆的差异阻断表明每个Ia分子存在一个以上的限制性位点。通过α和β链的组合结合或通过每个分子使用一个以上位点来增加可能的功能性Ia限制性位点的数量，应该会增加Ia分子在抗原呈递中发挥功能的方式的数量。
Monoclonal antibodies (mAb) were used to inhibit the proliferation of antigen-reactive (C57BL6/J X A/J)F1 restricted T cell clones. We have been able to subdivide these F1 restricted T cell clones into two groups: one of which recognizes the A alpha k A beta b molecule and the other group which recognizes the A alpha b A beta k molecule. Using clones with defined reactivities, we could assign the reactivities of monoclonals to the A alpha or A beta chains. By immunoprecipitation and two-dimensional analysis of Ia molecules from F1 spleen cells, we could independently map the reactivities of the mAb as being determined by the A alpha or A beta chain. To date, these two methods of chain localization of the antibody reactivity have agreed. Further, the differential blocking of the A alpha k A beta b restricted T cell clones suggests that there exists more than one restriction site per Ia molecule. Increasing the number of possible functional Ia restriction sites, either through combinatorial association of alpha and beta chains or by using more than one site per molecule, should increase the number of ways Ia molecules can function in antigen presentation.
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影响因子：
15.3
作者：
MATIS, LA;JONES, PP;SCHWARTZ, RH
通讯作者：
SCHWARTZ, RH
影响因子：
3.2
作者：
MCKEAN, DJ;MELVOLD, RW;DAVID, C
通讯作者：
DAVID, C
影响因子：
56.9
作者：
MCNICHOLAS, J;STEINMETZ, M;HOOD, L
通讯作者：
HOOD, L
影响因子：
15.3
作者：
MCNICHOLAS, JM;MURPHY, DB;JONES, PP
通讯作者：
JONES, PP
影响因子：
64.5
作者：
BENOIST, CO;MATHIS, DJ;MCDEVITT, HO
通讯作者：
MCDEVITT, HO