Hydrocarbon-Degrading Bacteria Alcanivorax and Marinobacter Associated With Microalgae Pavlova lutheri and Nannochloropsis oculata.
Hydrocarbon-Degrading Bacteria Alcanivorax and Marinobacter Associated With Microalgae Pavlova lutheri and Nannochloropsis oculata.
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与微藻pavlova lutheri和nannochloropsis oculata相关的碳氢化合物细菌和小杆菌。
DOI：
10.3389/fmicb.2020.572931
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发表时间：
2020
影响因子：
5.2
通讯作者：
Golyshin PN
中科院分区：
文献类型：
--
作者：
Chernikova TN;Bargiela R;Toshchakov SV;Shivaraman V;Lunev EA;Yakimov MM;Thomas DN;Golyshin PN
海洋碳氢化合物降解菌在天然石油生物降解过程中发挥着重要作用，最初与人为溢油或天然渗漏有关。然而，在没有石油的情况下，他们的自然生态位是什么并不完全清楚。很少有研究指出一些产生亲油化合物（烷烃，长链脂肪酸和醇）的海洋微藻是这些细菌的潜在天然宿主。我们建立了Dansk原油为基础的富集培养与光生物反应器生长的海洋微藻培养Pavlova lutheri和Nannochloropsis oculata，并使用培养和SSU（16 S）rRNA扩增子测序分析了微生物的演替。我们发现，石油在两种富集培养物中对α-和γ-变形菌的成员进行了强烈的选择，其中食烷菌属和海蛞蝓属普遍存在，著名的碳氢化合物细菌。总共分离并鉴定了48种非冗余细菌菌株，代表食烷菌属、海螺菌属、海旋菌属、丝单胞菌属、盐单胞菌属、海卵形菌属、玫瑰果菌属和油杆菌属，其在两种原油富集中的测序读段中丰富。我们的公共数据库的评估表明，一些重叠的地理位置的分离微拟球藻和巴夫洛娃的分子检测和分离的Alcanivorax和Marinoptera属。我们的研究表明，这些全球重要的碳氢化合物降解细菌与P. lutheri和N。有眼的
Marine hydrocarbon-degrading bacteria play an important role in natural petroleum biodegradation processes and were initially associated with man-made oil spills or natural seeps. There is no full clarity though on what, in the absence of petroleum, their natural niches are. Few studies pointed at some marine microalgae that produce oleophilic compounds (alkanes, long-chain fatty acids, and alcohols) as potential natural hosts of these bacteria. We established Dansk crude oil-based enrichment cultures with photobioreactor-grown marine microalgae cultures Pavlova lutheri and Nannochloropsis oculata and analyzed the microbial succession using cultivation and SSU (16S) rRNA amplicon sequencing. We found that petroleum enforced a strong selection for members of Alpha- and Gamma-proteobacteria in both enrichment cultures with the prevalence of Alcanivorax and Marinobacter spp., well-known hydrocarbonoclastic bacteria. In total, 48 non-redundant bacterial strains were isolated and identified to represent genera Alcanivorax, Marinobacter, Thalassospira, Hyphomonas, Halomonas, Marinovum, Roseovarius, and Oleibacter, which were abundant in sequencing reads in both crude oil enrichments. Our assessment of public databases demonstrated some overlaps of geographical sites of isolation of Nannochloropsis and Pavlova with places of molecular detection and isolation of Alcanivorax and Marinobacter spp. Our study suggests that these globally important hydrocarbon-degrading bacteria are associated with P. lutheri and N. oculata.
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影响因子：
15.5
作者：
Fadrosh DW;Ma B;Gajer P;Sengamalay N;Ott S;Brotman RM;Ravel J
通讯作者：
Ravel J
影响因子：
4.2
作者：
Green, DH;Llewellyn, LE;Bolch, CJS
通讯作者：
Bolch, CJS
影响因子：
64.8
作者：
Amin, S. A.;Hmelo, L. R.;Armbrust, E. V.
通讯作者：
Armbrust, E. V.
影响因子：
5.1
作者：
Cui Z;Lai Q;Dong C;Shao Z
通讯作者：
Shao Z
DOI：
10.1073/pnas.1000080107
发表时间：
2011-03-15
影响因子：
11.1
作者：
Caporaso, J. Gregory;Lauber, Christian L.;Knight, Rob
通讯作者：
Knight, Rob