Simultaneous characterization of instantaneous Young's modulus and specific membrane capacitance of single cells using a microfluidic system.
Simultaneous characterization of instantaneous Young's modulus and specific membrane capacitance of single cells using a microfluidic system.
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DOI：
10.3390/s150202763
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发表时间：
2015-01-27
期刊：
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Zhao Y;Chen D;Luo Y;Chen F;Zhao X;Jiang M;Yue W;Long R;Wang J;Chen J
本文提出了一种基于微流体的方法，能够连续表征悬浮单细胞的瞬时杨氏模量（Einstantaneous）和比膜电容（CSpecific membrane）。在该方法中，通过收缩通道吸出细胞，同时使用高速摄像机记录细胞进入收缩通道的过程，并通过锁定放大器同时测量两个频率（1 kHz 和 100 kHz）的阻抗分布。进行数值模拟来模拟细胞进入收缩通道的过程，重点关注两个关键参数：瞬时吸入长度（Linstantaneous）和过渡吸入长度（Ltransitional），后者进一步转化为瞬时。使用在收缩通道中移动的细胞的等效分布电路模型来确定C特异性膜。非小细胞肺癌细胞系 95C (n = 354) 用于评估该技术，产生 2.96 ± 0.40 kPa 的瞬时压力和 1.59 ± 0.28 μF/cm2 的 C 特异性膜。作为连续、同时表征细胞瞬时和 C 特异性膜的平台，该方法可以促进对细胞生物物理特性的更全面的了解。
This paper presents a microfluidics-based approach capable of continuously characterizing instantaneous Young's modulus (Einstantaneous) and specific membrane capacitance (Cspecific membrane) of suspended single cells. In this method, cells were aspirated through a constriction channel while the cellular entry process into the constriction channel was recorded using a high speed camera and the impedance profiles at two frequencies (1 kHz and 100 kHz) were simultaneously measured by a lock-in amplifier. Numerical simulations were conducted to model cellular entry process into the constriction channel, focusing on two key parameters: instantaneous aspiration length (Linstantaneous) and transitional aspiration length (Ltransitional), which was further translated to Einstantaneous. An equivalent distribution circuit model for a cell travelling in the constriction channel was used to determine Cspecific membrane. A non-small-cell lung cancer cell line 95C (n = 354) was used to evaluate this technique, producing Einstantaneous of 2.96 ± 0.40 kPa and Cspecific membrane of 1.59 ± 0.28 μF/cm2. As a platform for continuous and simultaneous characterization of cellular Einstantaneous and Cspecific membrane, this approach can facilitate a more comprehensive understanding of cellular biophysical properties.
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DOI：
10.1073/pnas.92.3.860
发表时间：
1995-01-31
影响因子：
11.1
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
通讯作者：
GASCOYNE, PRC
微流体系统能够连续表征悬浮中单细胞的比膜电容和细胞质电导率
影响因子：
12.6
作者：
Zhao, Yang;Chen, Deyong;Chen, Jian
通讯作者：
Chen, Jian
影响因子：
6.1
作者：
Zhao, Yang;Chen, Deyong;Chen, Jian
通讯作者：
Chen, Jian
影响因子：
1.3
作者：
Zheng, Yi;Sun, Yu
通讯作者：
Sun, Yu
影响因子：
6.1
作者：
Chen, Jian;Zheng, Yi;Sun, Yu
通讯作者：
Sun, Yu