Genomic and transcriptomic analysis of the AP2/ERF superfamily in Vitis vinifera.
Genomic and transcriptomic analysis of the AP2/ERF superfamily in Vitis vinifera.
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DOI：
10.1186/1471-2164-11-719
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发表时间：
2010-12-20
期刊：
影响因子：
4.4
通讯作者：
Perata P
中科院分区：
文献类型：
--
作者：
Licausi F;Giorgi FM;Zenoni S;Osti F;Pezzotti M;Perata P
AP 2/ERF蛋白家族包含在植物生长和发育以及植物对生物和非生物胁迫条件的响应中起关键作用的转录因子。葡萄藤（葡萄属vinifera）是唯一一种基因组已被完全测序的木本作物。到目前为止，还没有详细的葡萄AP 2/ERF样基因的表达谱。以葡萄属葡萄为材料，对AP 2/ERF基因进行了全基因组搜索，并采用实时荧光定量PCR（qRT-PCR）技术分析了其在不同营养组织和生殖组织中的表达谱。149个序列，含有至少一个ERF结构域，被确定。AP 2和ERF家族内的特定簇显示出保守的表达模式，让人想起其他物种和与浆果成熟相关的葡萄藤特定趋势。此外，通过共表达和蛋白质相似性比较确定了第IX组ERF的推定靶点。葡萄藤组含有大量的AP 2/ERF基因，与目前分析的其他双子叶植物物种相当。我们观察到ERF家族内特定群体的规模增加，可能是由于最近的重复事件。在不同的气生组织中的表达分析显示了先前在其他植物系统中描述的共同特征，并为一些ERF组成员在果实成熟过程中引入了可能的新作用。本研究为研究葡萄果实发育和成熟过程的分子调控奠定了基础。
The AP2/ERF protein family contains transcription factors that play a crucial role in plant growth and development and in response to biotic and abiotic stress conditions in plants. Grapevine (Vitis vinifera) is the only woody crop whose genome has been fully sequenced. So far, no detailed expression profile of AP2/ERF-like genes is available for grapevine. An exhaustive search for AP2/ERF genes was carried out on the Vitis vinifera genome and their expression profile was analyzed by Real-Time quantitative PCR (qRT-PCR) in different vegetative and reproductive tissues and under two different ripening stages. One hundred and forty nine sequences, containing at least one ERF domain, were identified. Specific clusters within the AP2 and ERF families showed conserved expression patterns reminiscent of other species and grapevine specific trends related to berry ripening. Moreover, putative targets of group IX ERFs were identified by co-expression and protein similarity comparisons. The grapevine genome contains an amount of AP2/ERF genes comparable to that of other dicot species analyzed so far. We observed an increase in the size of specific groups within the ERF family, probably due to recent duplication events. Expression analyses in different aerial tissues display common features previously described in other plant systems and introduce possible new roles for members of some ERF groups during fruit ripening. The presented analysis of AP2/ERF genes in grapevine provides the bases for studying the molecular regulation of berry development and the ripening process.
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