On the Mechanical Roles of Glycosaminoglycans in the Tensile Properties of Porcine Corneal Stroma.
On the Mechanical Roles of Glycosaminoglycans in the Tensile Properties of Porcine Corneal Stroma.
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DOI：
10.1167/iovs.64.4.3
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发表时间：
2023-04-03
影响因子：
4.4
通讯作者：
中科院分区：
文献类型：
--
作者：
角膜主要由嵌入在富含蛋白多糖和其他糖蛋白的基质中的胶原纤维组成。已知的糖胺多糖(GAG)侧链蛋白多糖在胶原纤维之间形成反平行的双链。本研究旨在探讨GAG在确定猪角膜基质拉伸特性中的力学功能。从鼻-颞方向解剖猪角膜基质条，分为对照组、缓冲液处理组和酶处理组。对照组标本在解剖后即刻取材。然而，缓冲液处理和酶处理的样品分别在由100 mM醋酸钠组成的缓冲液中(pH 6.0)和在含有角聚糖酶II的酶溶液中在37℃下孵育18h。Blycan法测定酶和缓冲液处理的样品中的总GAG含量并评估GAG消耗。还进行了单轴拉伸试验，以确定去除GAG对角膜力学性能的影响。酶处理组GAG含量显著低于正常组和缓冲液处理组(P<；0.05)。此外，与对照和缓冲液样本相比，去除GAG的试条的机械反应明显较软(P<；0.05)。去除角膜细胞外基质中的GAG可显著降低角膜基质的拉伸性能；支持GAG含量与角膜基质力学性能之间存在很强相关性的假设。
The cornea is primarily composed of collagen fibrils that are embedded in a ground substance rich in proteoglycans and other glycoproteins. It is known that glycosaminoglycan (GAG) side chains of proteoglycans form anti-parallel duplexes between collagen fibrils. The present work was done in order to investigate the mechanical function of GAGs in defining the tensile properties of porcine corneal stroma. Porcine corneal stromal strips dissected from the nasal–temporal direction were divided into control, buffer-treated, and enzyme-treated groups. The samples in the control group were used immediately after dissection. However, the buffer-treated and enzyme-treated samples were, respectively, incubated for 18 hours at 37°C in a buffer solution made up of 100-mM sodium acetate at pH 6.0 or in an enzyme solution containing keratanase II. The Blyscan assay was used to quantify the total GAG content and assess GAG depletion in the samples treated with the enzyme and buffer solutions. Uniaxial tensile tests were also performed to determine the effect of GAG removal on mechanical properties of the cornea. The GAG content in enzyme-treated samples was significantly lower than that of the normal and buffer-treated specimens (P < 0.05). Moreover, GAG-depleted strips showed significantly softer mechanical responses in comparison with the control and buffer samples (P < 0.05). Removing GAGs from the corneal extracellular matrix led to significant tensile property reduction; supporting the hypothesis that there exists a strong correlation between the GAG content and mechanical properties of the corneal stroma.
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影响因子：
3.3
作者：
Chaerkady R;Shao H;Scott SG;Pandey A;Jun AS;Chakravarti S
通讯作者：
Chakravarti S
DOI：
10.1073/pnas.2116718119
发表时间：
2022-04-12
影响因子：
11.1
作者：
通讯作者：
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影响因子：
3.4
作者：
Boubriak, OA;Urban, JPG;Bron, AJ
通讯作者：
Bron, AJ
影响因子：
64.8
作者：
CLOWES, AW;KARNOWSKY, MJ
通讯作者：
KARNOWSKY, MJ
影响因子：
3.4
作者：
ANDREASSEN, TT;SIMONSEN, AH;OXLUND, H
通讯作者：
OXLUND, H