Sex differences in spinal processing of transient and inflammatory colorectal stimuli in the rat.
Sex differences in spinal processing of transient and inflammatory colorectal stimuli in the rat.
复制标题
大鼠瞬时和炎症性结直肠刺激的脊柱加工性别差异。
DOI：
10.1016/j.pain.2012.06.019
复制
发表时间：
2012-09
期刊：
影响因子：
7.4
通讯作者：
Traub RJ
中科院分区：
文献类型：
--
作者：
Ji Y;Tang B;Cao DY;Wang G;Traub RJ
在躯体和内脏刺激的脊椎处理过程中的性别差异导致女性在许多疼痛障碍中更敏感。本研究考察了导致内脏敏感性性别差异的脊椎机制。与雄性大鼠相比，正常雌性大鼠和使用芥子油后的正常雌性大鼠对结肠扩张(CRD)的内脏运动反应更强烈。正常大鼠和芥子油处理组大鼠的腰臀(LS)和胸腰段(TL)结肠传入的CRD诱发反应没有性别差异，但自发活动存在性别差异，而灌胃芥子油则加剧了这一反应。正常女性LS和TL脊髓节段的内脏感受性背角神经元对CRD的反应较大。结肠内芥子油对不同表型内脏感受性背角神经元CRD诱发反应的影响具有性别和节段依赖性。NMDA受体拮抗剂APV呈剂量依赖性地减弱正常大鼠的内脏运动反应，对雄性大鼠的影响更大。相应地，雌性大鼠背角提取液中GluN1亚单位的细胞膜表达水平较高。使用芥子油后，在LS节段APV和GluN1表达的影响不再存在性别差异，但在TL节段女性表达更多。这些数据证明了正常结肠和发炎结肠在脊髓处理伤害性内脏刺激方面的性别差异。在清醒大鼠观察到的内脏敏感性方面，背角神经元活动和NMDA受体表达的差异是性别差异的原因之一。
Sex differences in the spinal processing of somatic and visceral stimuli contribute to greater female sensitivity in many pain disorders. The present study examined spinal mechanisms that contribute to sex differences in visceral sensitivity. The visceromotor response to colorectal distention (CRD) was more robust in normal female rats and following intracolonic mustard oil compared to males. No sex difference was observed in the CRD-evoked response of lumbosacral (LS) and thoracolumbar (TL) colonic afferents in normal and mustard oil-treated rats, but there was a sex difference in spontaneous activity that was exacerbated by intracolonic mustard oil. The response of visceroceptive dorsal horn neurons to CRD was greater in normal females in the LS and TL spinal segments. The effect of intracolonic mustard oil on the CRD-evoked response of different phenotypes of visceroceptive dorsal horn neurons was dependent on sex and segment. The NMDA receptor antagonist APV dose-dependently attenuated the visceromotor response in normal rats with greater effect in males. Correspondingly, there was greater cell membrane expression of the GluN1 subunit in dorsal horn extracts in females. Following intracolonic mustard oil there was no longer a sex difference in the effect of APV nor GluN1 expression in LS segments but greater female expression in TL segments. These data document a sex difference in spinal processing of nociceptive visceral stimuli from the normal and inflamed colon. Differences in dorsal horn neuronal activity and NMDA receptor expression contribute to the sex differences in the visceral sensitivity observed in awake rats.
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DOI：
10.1152/ajpregu.00824.2005
发表时间：
2006-08-01
影响因子：
2.8
作者：
Ji, Yaping;Murphy, Anne Z.;Traub, Richard J.
通讯作者：
Traub, Richard J.
影响因子：
2.5
作者：
Heitkemper, Margaret;Jarrett, Monica;Chang, Lin
通讯作者：
Chang, Lin
影响因子：
3.3
作者：
Ji, Y.;Tang, B.;Traub, R. J.
通讯作者：
Traub, R. J.
影响因子：
8.8
作者：
HAWKINS, RA;FREEDMAN, B;KILLEN, E
通讯作者：
KILLEN, E
影响因子：
2.5
作者：
AlChaer, ED;Lawand, NB;Willis, WD
通讯作者：
Willis, WD