Molecular motor MYO1C, acetyltransferase KAT6B and osteogenetic transcription factor RUNX2 expression in human masseter muscle contributes to development of malocclusion.
Molecular motor MYO1C, acetyltransferase KAT6B and osteogenetic transcription factor RUNX2 expression in human masseter muscle contributes to development of malocclusion.
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DOI：
10.1016/j.archoralbio.2014.03.005
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发表时间：
2014-06
影响因子：
3
通讯作者：
Sciote, James J.
中科院分区：
文献类型：
--
作者：
Desh, Heather;Gray, S. Lauren;Horton, Michael J.;Raoul, Gwenael;Rowlerson, Anthea M.;Ferri, Joel;Vieira, Alexandre R.;Sciote, James J.
I型肌球蛋白是细胞质中葡萄糖转运和细胞核中转录起始所必需的分子马达。其中两个，MYO 1H和MYO 1C，是旁系同源物，这可能是重要的发展错。本研究的目的是探讨其基因表达在咬合不正的人咬肌。还研究了两种已知有助于错牙合的功能相关蛋白：KAT 6 B（一种由MYO 1C激活的染色质重塑表观遗传酶）和RUNX 2（一种由KAT 6 B激活的调节骨生成的转录因子）。咬肌样本和错牙合分类从正颌外科受试者获得。将肌肉切片并免疫染色以确定纤维类型特性。从剩余的样品中分离RNA以通过TaqMan® RT-PCR测定四种基因的表达水平。比较两组牙弓纤维类型、基因表达量及错牙合分类的差异。MYO 1C和KAT 6 B的表达有非常显著的相关性（P<0.000001）。RUNX 2表达与咬肌II型纤维特性之间也存在显著相关性（P<0.005）。MYO 1C和咬肌纤维类型特性之间几乎没有显著的关联。MYO 1C和KAT 6 B之间的关系表明，两者在基因表达的染色质重塑中相互作用。这是MYO 1C的核肌球蛋白1（NM 1）功能。一个令人惊讶的发现是RUNX 2和II型咬肌纤维之间的关系，因为RUNX 2在成熟肌肉中的表达以前是未知的。RUNX 2在成人咬肌中的作用有待进一步研究。
Type I myosins are molecular motors necessary for glucose transport in the cytoplasm and initiation of transcription in the nucleus. Two of these, MYO1H and MYO1C, are paralogs which may be important in the development of malocclusion. The objective of this study was to investigate their gene expression in the masseter muscle of malocclusion subjects. Two functionally related proteins known to contribute to malocclusion were also investigated: KAT6B (a chromatin remodeling epigenetic enzyme which is activated by MYO1C) and RUNX2 (a transcription factor regulating osteogenesis which is activated by KAT6B). Masseter muscle samples and malocclusion classifications were obtained from orthognathic surgery subjects. Muscle was sectioned and immunostained to determine fiber type properties. RNA was isolated from the remaining sample to determine expression levels for the four genes by TaqMan® RT-PCR. Fiber type properties, gene expression quantities and malocclusion classification were compared. There were very significant associations (P<0.0000001) between MYO1C and KAT6B expressions. There were also significant associations (P<0.005) between RUNX2 expression and masseter muscle type II fiber properties. Very few significant associations were identified between MYO1C and masseter muscle fiber type properties. The relationship between MYO1C and KAT6B suggests that the two are interacting in chromatin remodeling for gene expression. This is the nuclear myosin1 (NM1) function of MYO1C. A surprising finding is the relationship between RUNX2 and type II masseter muscle fibers, since RUNX2 expression in mature muscle was previously unknown. Further investigations are necessary to elucidate the role of RUNX2 in adult masseter muscle.
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影响因子：
56.9
作者：
Laakso, Joseph M.;Lewis, John H.;Shuman, Henry;Ostap, E. Michael
通讯作者：
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