Antisense-mediated suppression of tomato zeaxanthin epoxidase alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance

Antisense-mediated suppression of tomato zeaxanthin epoxidase alleviates photoinhibition of PSII and PSI during chilling stress under low irradiance
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反义介导的番茄玉米黄质环氧化酶抑制可减轻低辐照度低温胁迫期间 PSII 和 PSI 的光抑制

DOI:
10.1007/s11099-010-0053-0
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发表时间:
2010-10
期刊:
影响因子:
2.7
通讯作者:
--
中科院分区:
生物学4区
文献类型:
--
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番茄(Lycopersicon esculentumMill.)分离了玉米黄质环氧酶基因(LeZE),并获得了反义转基因番茄植株。北方、南方和westernblot分析表明,反义LeZE基因已转入番茄基因组,其表达受到抑制。在强光和低温胁迫下,反义转基因植株的(A+Z)/(V+A+Z)比值维持在高于野生型(WT)的水平。野生型和转基因植物的非光化学猝灭(NPQ)值在胁迫期间不受影响。转基因植株的可氧化P700和PSII最大光化学效率(Fv/Fm)在低温低光照下下降较慢。这些结果表明,抑制LeZE的表达可以促进玉米黄质的积累,从而缓解低温胁迫下番茄PSI和PSII的光抑制。
A tomato (Lycopersicon esculentumMill.) zeaxanthin epoxidase gene (LeZE) was isolated and antisense transgenic tomato plants were produced. Northern, southern, and western blot analyses demonstrated that antisenseLeZEwas transferred into the tomato genome and the expression ofLeZEwas inhibited. The ratio of (A+Z)/(V+A+Z) in antisense transgenic plants was maintained at a higher level than in the wild type (WT) plants under high light and chilling stress with low irradiance. The value of non-photochemical quenching (NPQ) in WT and transgenic plants was not affected during the stresses. The oxidizable P700 and the maximal photochemical efficiency of PSII (Fv/Fm) in transgenic plants decreased more slowly at chilling temperature under low irradiance. These results suggested that suppression ofLeZEcaused zeaxanthin accumulation, which was helpful in alleviating photoinhibition of PSI and PSII in tomato plants under chilling stress.
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