Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes.
Comparison of the Venom Peptides and Their Expression in Closely Related Conus Species: Insights into Adaptive Post-speciation Evolution of Conus Exogenomes.
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DOI：
10.1093/gbe/evv109
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发表时间：
2015-06-04
影响因子：
3.3
通讯作者：
Lluisma AO
中科院分区：
文献类型：
--
作者：
Barghi N;Concepcion GP;Olivera BM;Lluisma AO
编码具有外源靶标的产物的基因（其构成生物体的“外源基因组”）通常表现出高进化速率。广义芋螺中编码毒液肽（芋螺毒素或芋螺肽）的基因属于这类基因。它们的快速多样化已经确立，并被认为与该属的高物种形成率有关。然而，毒液肽多样化和新物种最终出现的分子机制仍然知之甚少。在这项研究中，芋螺毒素的序列和表达水平从两个密切相关的蠕虫狩猎物种，芋螺tribblei和芋螺lenavati，几个标本进行了比较，通过转录组分析。大多数确定的推定conopeptides是新的，它们的多样性，即使在每个标本，是非常高的，这表明了广泛的猎物目标，这些物种。芋螺肽在超家族水平的种间表达模式的比较导致发现保守以及物种特异性的表达模式，表明在影响芋螺毒素基因表达的调控网络的分歧。个别蜗牛的转录组的比较显示，每个标本产生一个独特的一组高度表达的conopeptides，反映了个别蜗牛微调其毒液的组成的能力。这些观察结果反映了序列分歧和分歧的作用，在控制特定的芋螺肽的表达在进化的外基因组，因此毒液组成芋螺。
Genes that encode products with exogenous targets, which comprise an organism's “exogenome,” typically exhibit high rates of evolution. The genes encoding the venom peptides (conotoxins or conopeptides) in Conus sensu lato exemplify this class of genes. Their rapid diversification has been established and is believed to be linked to the high speciation rate in this genus. However, the molecular mechanisms that underlie venom peptide diversification and ultimately emergence of new species remain poorly understood. In this study, the sequences and expression levels of conotoxins from several specimens of two closely related worm-hunting species, Conus tribblei and Conus lenavati, were compared through transcriptome analysis. Majority of the identified putative conopeptides were novel, and their diversity, even in each specimen, was remarkably high suggesting a wide range of prey targets for these species. Comparison of the interspecific expression patterns of conopeptides at the superfamily level resulted in the discovery of both conserved as well as species-specific expression patterns, indicating divergence in the regulatory network affecting conotoxin gene expression. Comparison of the transcriptomes of the individual snails revealed that each specimen produces a distinct set of highly expressed conopeptides, reflecting the capability of individual snails to fine-tune the composition of their venoms. These observations reflect the role of sequence divergence and divergence in the control of expression for specific conopeptides in the evolution of the exogenome and hence venom composition in Conus.
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影响因子：
3
作者：
Davis, Jasmine;Jones, Alun;Lewis, Richard J.
通讯作者：
Lewis, Richard J.
影响因子：
10.7
作者：
Duda, TF;Palumbi, SR
通讯作者：
Palumbi, SR
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4.9
作者：
Duda, T. F., Jr.;Remigio, E. A.
通讯作者：
Remigio, E. A.
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作者：
Chang, Dan;Duda, Thomas F., Jr.
通讯作者：
Duda, Thomas F., Jr.
影响因子：
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作者：
Dutertre, Sebastien;Biass, Daniel;Favreau, Philippe
通讯作者：
Favreau, Philippe