Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper.
Comparative genomics reveals diversity among xanthomonads infecting tomato and pepper.
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比较基因组学揭示了感染番茄和胡椒粉的黄金多诺德人的多样性。
DOI：
10.1186/1471-2164-12-146
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发表时间：
2011-03-11
期刊：
影响因子：
4.4
通讯作者：
Jones JB
中科院分区：
文献类型：
--
作者：
Potnis N;Krasileva K;Chow V;Almeida NF;Patil PB;Ryan RP;Sharlach M;Behlau F;Dow JM;Momol M;White FF;Preston JF;Vinatzer BA;Koebnik R;Setubal JC;Norman DJ;Staskawicz BJ;Jones JB
番茄和辣椒的细菌性斑点病是由四种黄单胞菌引起的，是温暖潮湿气候下的一种主要植物病害。这四个物种在生理和分子特征上彼此不同。85-10菌株的基因组序列已被报道，该菌株属于黄单胞菌（Xanthomonaseuvesicatoria，Xcv）。为了在基因组水平上确定这四个物种的关系，并研究它们的毒力和不同宿主范围的分子基础，确定了其他三个物种成员的基因组序列草案，并与菌株85-10进行了比较。我们对X染色体组进行了测序。vesicatoria（Xv）菌株1111（ATCC 35937）、X.穿孔素（Xp）菌株91-118和X. gardneri（Xg）菌株101（ATCC 19865）。将基因组相互比较，并与先前测序的Xcv菌株85-10进行比较。此外，预测致病性可能需要的分子特征，包括III型分泌器，III型效应器，其他分泌系统，群体感应系统，粘附素，胞外多糖和脂多糖决定簇。计算鉴定了Xg菌株101和Xv菌株1111基因组中的几种新型III型效应子，并使用报告基因测定验证了它们的易位。在Xcv中鉴定了一个Ax 21的同源物，该同源物是XA 21介导的水稻抗性的激发子，并且鉴定了一个功能性Ax 21硫酸化系统。还比较了编码具有由II型和IV型分泌系统介导的功能的蛋白质的基因，包括参与细胞壁解构的酶，作为致病性的贡献者。比较基因组分析揭示了相当大的细菌斑点病原体之间的多样性，提供了新的见解的差异和相似性，可以解释这些菌株的多样性。辣椒病原体的特异性基因，如脂多糖簇的O-抗原，以及个别菌株特有的基因，如新型III型效应子和细菌素基因，已被确定为我们了解病原体的毒力，侵略性和宿主偏好提供了新的线索。这些分析将有助于在经济上重要的番茄和辣椒品种的广泛和持久的抗性育种的努力。
Bacterial spot of tomato and pepper is caused by four Xanthomonas species and is a major plant disease in warm humid climates. The four species are distinct from each other based on physiological and molecular characteristics. The genome sequence of strain 85-10, a member of one of the species, Xanthomonas euvesicatoria (Xcv) has been previously reported. To determine the relationship of the four species at the genome level and to investigate the molecular basis of their virulence and differing host ranges, draft genomic sequences of members of the other three species were determined and compared to strain 85-10. We sequenced the genomes of X. vesicatoria (Xv) strain 1111 (ATCC 35937), X. perforans (Xp) strain 91-118 and X. gardneri (Xg) strain 101 (ATCC 19865). The genomes were compared with each other and with the previously sequenced Xcv strain 85-10. In addition, the molecular features were predicted that may be required for pathogenicity including the type III secretion apparatus, type III effectors, other secretion systems, quorum sensing systems, adhesins, extracellular polysaccharide, and lipopolysaccharide determinants. Several novel type III effectors from Xg strain 101 and Xv strain 1111 genomes were computationally identified and their translocation was validated using a reporter gene assay. A homolog to Ax21, the elicitor of XA21-mediated resistance in rice, and a functional Ax21 sulfation system were identified in Xcv. Genes encoding proteins with functions mediated by type II and type IV secretion systems have also been compared, including enzymes involved in cell wall deconstruction, as contributors to pathogenicity. Comparative genomic analyses revealed considerable diversity among bacterial spot pathogens, providing new insights into differences and similarities that may explain the diverse nature of these strains. Genes specific to pepper pathogens, such as the O-antigen of the lipopolysaccharide cluster, and genes unique to individual strains, such as novel type III effectors and bacteriocin genes, have been identified providing new clues for our understanding of pathogen virulence, aggressiveness, and host preference. These analyses will aid in efforts towards breeding for broad and durable resistance in economically important tomato and pepper cultivars.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
3.5
作者：
BONAS, U;SCHULTE, R;STALL, RE
通讯作者：
STALL, RE
影响因子：
3.5
作者：
Buettner, Daniela;Noel, Laurent;Bonas, Ulla
通讯作者：
Bonas, Ulla
影响因子：
3.2
作者：
Bouzar, H;Jones, JB;Jackson, LE
通讯作者：
Jackson, LE
影响因子：
12.3
作者：
Crossman LC;Gould VC;Dow JM;Vernikos GS;Okazaki A;Sebaihia M;Saunders D;Arrowsmith C;Carver T;Peters N;Adlem E;Kerhornou A;Lord A;Murphy L;Seeger K;Squares R;Rutter S;Quail MA;Rajandream MA;Harris D;Churcher C;Bentley SD;Parkhill J;Thomson NR;Avison MB
通讯作者：
Avison MB