Spiking patterns and their functional implications in the antennal lobe of the tobacco hornworm Manduca sexta.
Spiking patterns and their functional implications in the antennal lobe of the tobacco hornworm Manduca sexta.
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DOI：
10.1371/journal.pone.0023382
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hildebrand JG
中科院分区：
文献类型：
--
作者：
Lei H;Reisenman CE;Wilson CH;Gabbur P;Hildebrand JG
爆发式和紧张式放电模式在各种感觉系统中都有描述。在嗅觉系统中，已经观察到分布在几个突触水平的神经元自发爆发，从外周到嗅球(OB)和嗅皮层。几项体外研究表明，自发放电模式可能被视为不同类型神经元的“指纹”，这些神经元在OB中表现出不同的功能。然而，神经元的爆发性是否以及如何与自然嗅觉刺激的编码相关仍不清楚。因此，我们进行了一项活体研究，以探索这个问题在昆虫的OB相当结构，烟草角虫的触角叶(AL)。我们发现，在蛾的AL中，投射(输出)神经元(PNS)和局部中间神经元(LNS)都是自发活动的，但PNS倾向于产生棘波爆发，而LNS的放电更有规律。此外，我们还发现，三叉神经核的突发性与其对气味刺激的反应强度有关--突发性越强，对气味的反应越强。此外，PNS的爆发性也与神经元的自发放电率呈正相关，药物对爆发性的抑制导致神经元的反应性降低。这些结果表明，神经元的爆发性反映了这些神经元的一种生理状态，这与它们的反应特征直接相关。
Bursting as well as tonic firing patterns have been described in various sensory systems. In the olfactory system, spontaneous bursts have been observed in neurons distributed across several synaptic levels, from the periphery, to the olfactory bulb (OB) and to the olfactory cortex. Several in vitro studies indicate that spontaneous firing patterns may be viewed as “fingerprints” of different types of neurons that exhibit distinct functions in the OB. It is still not known, however, if and how neuronal burstiness is correlated with the coding of natural olfactory stimuli. We thus conducted an in vivo study to probe this question in the OB equivalent structure of insects, the antennal lobe (AL) of the tobacco hornworm Manduca sexta. We found that in the moth's AL, both projection (output) neurons (PNs) and local interneurons (LNs) are spontaneously active, but PNs tend to produce spike bursts while LNs fire more regularly. In addition, we found that the burstiness of PNs is correlated with the strength of their responses to odor stimulation – the more bursting the stronger their responses to odors. Moreover, the burstiness of PNs was also positively correlated with the spontaneous firing rate of these neurons, and pharmacological reduction of bursting resulted in a decrease of the neurons' responsiveness. These results suggest that neuronal burstiness reflects a physiological state of these neurons that is directly linked to their response characteristics.
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影响因子：
2.5
作者：
LEGENDY, CR;SALCMAN, M
通讯作者：
SALCMAN, M
影响因子：
3.3
作者：
Hamilton, KA;Heinbockel, T;Hayar, A
通讯作者：
Hayar, A
DOI：
10.1007/bf00611929
发表时间：
1987-01-01
影响因子：
2.1
作者：
CHRISTENSEN, TA;HILDEBRAND, JG
通讯作者：
HILDEBRAND, JG
影响因子：
2.5
作者：
Mercer, AR;Hildebrand, JG
通讯作者：
Hildebrand, JG
影响因子：
2.2
作者：
CHRISTENSEN, TA;HILDEBRAND, JG;DOOLITTLE, RE
通讯作者：
DOOLITTLE, RE