Human Adaptation of Ebola Virus during the West African Outbreak.
Human Adaptation of Ebola Virus during the West African Outbreak.
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DOI：
10.1016/j.cell.2016.10.013
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发表时间：
2016-11-03
期刊：
影响因子：
64.5
通讯作者：
Ball, Jonathan K.
中科院分区：
文献类型：
--
作者：
Urbanowicz, Richard A.;McClure, C. Patrick;Sakuntabhai, Anavaj;Sall, Amadou A.;Kobinger, Gary;Mueller, Marcel A.;Holmes, Edward C.;Rey, Felix A.;Simon-Loriere, Etienne;Ball, Jonathan K.
2013-2016 年西非爆发的埃博拉病毒（EBOV）是有记录以来规模最大的一次。它是在埃博拉病毒从动物宿主（很可能是蝙蝠）跨物种传播到人类之后开始的，系统发育分析揭示了几种病毒谱系的共同循环。我们假设，这种延长的人体循环导致了基因组变化，从而增加了病毒在人类中的传播能力。我们根据最早报道的分离株生成了合成糖蛋白（GP）构建体，并引入了定义病毒谱系的氨基酸取代。突变的 GP 用于生成一组假病毒，用于感染不同的人类和蝙蝠细胞系。这些数据表明，EBOV GP 中的特定氨基酸取代增加了对人类细胞的向性，同时降低了对蝙蝠细胞的向性。这种传染性的增加可能增强了埃博拉病毒在人类之间传播的能力，并导致一些病毒谱系的广泛地理分布。西非疫情期间埃博拉病毒适应了人类 埃博拉病毒糖蛋白中的氨基酸取代增加了对人类细胞的向性 相同的糖蛋白氨基酸取代降低了对蝙蝠细胞的向性 埃博拉病毒在其糖蛋白中获得了氨基酸取代，这在 2013-2016 年西非疫情期间增加了其对人类细胞的向性。
The 2013–2016 outbreak of Ebola virus (EBOV) in West Africa was the largest recorded. It began following the cross-species transmission of EBOV from an animal reservoir, most likely bats, into humans, with phylogenetic analysis revealing the co-circulation of several viral lineages. We hypothesized that this prolonged human circulation led to genomic changes that increased viral transmissibility in humans. We generated a synthetic glycoprotein (GP) construct based on the earliest reported isolate and introduced amino acid substitutions that defined viral lineages. Mutant GPs were used to generate a panel of pseudoviruses, which were used to infect different human and bat cell lines. These data revealed that specific amino acid substitutions in the EBOV GP have increased tropism for human cells, while reducing tropism for bat cells. Such increased infectivity may have enhanced the ability of EBOV to transmit among humans and contributed to the wide geographic distribution of some viral lineages. EBOV adapted to humans during the West African outbreak Amino acid substitutions in the EBOV glycoprotein increase human cell tropism The same glycoprotein amino acid substitutions decrease tropism for bat cells The Ebola virus acquired amino acid substitutions in its glycoprotein that increased its tropism for human cells during the West African outbreak of 2013–2016.
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影响因子：
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作者：
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影响因子：
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DOI：
10.1126/science.1259657
发表时间：
2014-09-12
期刊：
Science (New York, N.Y.)
影响因子：
--
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Gire SK;Goba A;Andersen KG;Sealfon RS;Park DJ;Kanneh L;Jalloh S;Momoh M;Fullah M;Dudas G;Wohl S;Moses LM;Yozwiak NL;Winnicki S;Matranga CB;Malboeuf CM;Qu J;Gladden AD;Schaffner SF;Yang X;Jiang PP;Nekoui M;Colubri A;Coomber MR;Fonnie M;Moigboi A;Gbakie M;Kamara FK;Tucker V;Konuwa E;Saffa S;Sellu J;Jalloh AA;Kovoma A;Koninga J;Mustapha I;Kargbo K;Foday M;Yillah M;Kanneh F;Robert W;Massally JL;Chapman SB;Bochicchio J;Murphy C;Nusbaum C;Young S;Birren BW;Grant DS;Scheiffelin JS;Lander ES;Happi C;Gevao SM;Gnirke A;Rambaut A;Garry RF;Khan SH;Sabeti PC
通讯作者：
Sabeti PC