BAC library for the amphipod crustacean, Parhyale hawaiensis.
BAC library for the amphipod crustacean, Parhyale hawaiensis.
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DOI：
10.1016/j.ygeno.2010.03.005
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发表时间：
2010-05
期刊：
影响因子：
4.4
通讯作者：
Patel, Nipam H.
中科院分区：
文献类型：
--
作者：
Parchem, Ronald J.;Poulin, Francis;Stuart, Andrew B.;Amemiya, Chris T.;Patel, Nipam H.
细菌人工染色体（BACs）具有繁殖大片段DNA的能力，已成为研究基因组生物学的宝贵工具。为了填补现有基因组序列的系统发育空白，并补充正在进行的分子和遗传学研究，我们建立了海洋片脚甲壳类动物夏威夷对虾的BAC文库。该文库是从整个成年动物的基因组DNA中分离出来的，包括129024个个体克隆。中位数插入大小约为140 kb，基因组覆盖率约为5个基因组当量。我们筛选了Parhyale BAC文库中与发育相关的基因，并鉴定了四个基因的基因组组织：一个刺猬同源基因和三个Pax3/7同源基因。初步分析表明，内含子在Parhyale中比在其他已测序的节肢动物中更大更普遍，这可能部分解释了Parhyale基因组大的原因。
Bacterial artificial chromosomes (BACs) are capable of propagating large fragments of DNA and have become an invaluable tool for studying genome biology. To fill a phylogenetic gap in available genomic sequence and to complement ongoing molecular and genetic studies, we have generated a BAC library for the marine amphipod crustacean, Parhyale hawaiensis. The library was generated from genomic DNA isolated from whole adult animals and comprises 129,024 individual clones. The median insert size is ~140 kb and the genomic coverage is approximately five genome equivalents. We have screened the Parhyale BAC library for developmentally relevant genes and characterized the genomic organization of four genes: a hedgehog ortholog, and three Pax3/7 paralogs. Preliminary analysis suggests that introns are larger and more prevalent in Parhyale than in other arthropods whose genomes have been sequenced, which may partly account for the large genome size in Parhyale.
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