Lacticaseibacillus paracasei PS23 Effectively Modulates Gut Microbiota Composition and Improves Gastrointestinal Function in Aged SAMP8 Mice.
Lacticaseibacillus paracasei PS23 Effectively Modulates Gut Microbiota Composition and Improves Gastrointestinal Function in Aged SAMP8 Mice.
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DOI：
10.3390/nu13041116
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发表时间：
2021-03-29
期刊：
影响因子：
5.9
通讯作者：
Huang HY
中科院分区：
文献类型：
--
作者：
Chen LH;Wang MF;Chang CC;Huang SY;Pan CH;Yeh YT;Huang CH;Chan CH;Huang HY
据报道，益生菌通过调节肠道微生物区系来改善胃肠道(GI)功能。然而，益生菌是如何影响老年人的GM和GI功能的，目前还没有得到很好的描述。因此，在本研究中，我们观察了益生菌副乳杆菌PS23(LPPS23)对老龄小鼠GM和GI功能的影响。实验分为4组：未治疗对照组、生理盐水对照组、低剂量LPPS23组(1×108个克隆形成单位/只/天)和高剂量LPPS23组(1×109CFU/只/天)。未处理的小鼠在16周龄时被安乐死，其他小鼠在28周龄时被安乐死。下一代测序结果显示，LPPS23富含乳杆菌和假丝酵母菌，而乳螺科UCG_001丰度在给予LPPS23的老龄小鼠中降低。乳酸菌的丰度与白藜芦科的丰度呈负相关。此外，由于LPPS23处理的小鼠具有较长的肠道长度、较低的肠道通透性和较高的吞噬能力，LPPS23改善了老年小鼠的胃肠功能。酶联免疫吸附试验结果显示，LPPS23可减轻炎症因子和免疫球蛋白的变化。Spearman相关分析显示，富含LPPS23的乳酸菌丰度与老年小鼠的健康胃肠功能呈正相关，而LPPS23抑制的乳酸菌UCG_001与老年小鼠的健康GI功能呈负相关。综上所述，LPPS23能有效调节老龄SAMP8小鼠的GM成分，改善GI功能。
Probiotics are reported to improve gastrointestinal (GI) function via regulating gut microbiota (GM). However, exactly how probiotics influence GM and GI function in elders is poorly characterized. Therefore, in this study, we assessed the effect of the probiotic Lacticaseibacillus paracasei PS23 (LPPS23) on the GM and GI function of aged mice. There were four groups of senescence-accelerated mouse prone-8 (SAMP8) mice (n = 4): a non-treated control group, a saline control group, a low dose LPPS23 group (1 × 108 colony-forming unit (CFU)/mouse/day), and a high dose LPPS23 group (1 × 109 CFU/mouse/day). Non-treated mice were euthanized at 16 weeks old, and others were euthanized at 28 weeks old. The next-generation sequencing results revealed that LPPS23 enriched Lactobacillus and Candidatus_Saccharimonas, while the abundance of Lachnospiraceae_UCG_001 decreased in aged mice given LPPS23. The abundance of Lactobacillus negatively correlated with the abundance of Erysipelotrichaceae. Moreover, LPPS23 improved the GI function of aged mice due to the longer intestine length, lower intestinal permeability, and higher phagocytosis in LPPS23-treated mice. The ELISA results showed that LPPS23 attenuated the alterations of pro-inflammatory factors and immunoglobulins. The abundance of LPPS23-enriched Lactobacillus was positively correlated with healthy GI function, while Lachnospiraceae_UCG_001, which was repressed by LPPS23, was negatively correlated with a healthy GI function in the aged mice according to Spearman’s correlation analysis. Taken together, LPPS23 can effectively modulate GM composition and improve GI function in aged SAMP8 mice.
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影响因子：
4.6
作者：
Annaert, Pieter;Brouwers, Joachim;Augustijns, Patrick
通讯作者：
Augustijns, Patrick
影响因子：
3.7
作者：
Biagi E;Nylund L;Candela M;Ostan R;Bucci L;Pini E;Nikkïla J;Monti D;Satokari R;Franceschi C;Brigidi P;De Vos W
通讯作者：
De Vos W
影响因子：
5.7
作者：
Li HL;Lu L;Wang XS;Qin LY;Wang P;Qiu SP;Wu H;Huang F;Zhang BB;Shi HL;Wu XJ
通讯作者：
Wu XJ
影响因子：
3.5
作者：
HOLLANDER, D;TARNAWSKI, H
通讯作者：
TARNAWSKI, H
影响因子：
5.8
作者：
Malaguarnera, G.;Leggio, F.;Salmeri, M.
通讯作者：
Salmeri, M.