Transcriptional Analysis of Coccidioides immitis Mycelia and Spherules by RNA Sequencing.
Transcriptional Analysis of Coccidioides immitis Mycelia and Spherules by RNA Sequencing.
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DOI：
10.3390/jof7050366
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发表时间：
2021-05-07
期刊：
影响因子：
--
通讯作者：
Kirkland TN
中科院分区：
文献类型：
--
作者：
Carlin AF;Beyhan S;Peña JF;Stajich JE;Viriyakosol S;Fierer J;Kirkland TN
球孢子虫和球孢子虫是一种二相性真菌，从土壤中带有内节孢子的菌丝体转化为哺乳动物中被称为球体的组织形式。通过提高温度、CO2浓度和改变其他培养条件，可以在体外重现这一过程。在这项研究中，我们分析了菌丝体和幼龄和成熟球体中基因表达的变化。在年轻的球体中高度上调的基因包括球体表面蛋白以及铁和铜膜转运蛋白。球孢子虫特有的基因。在这个组中也有过多的表达，这表明它们对球体分化可能是重要的。在年轻的球体上调的基因中，丰富的GO术语包括氧化还原、对胁迫的反应和膜蛋白。下调的基因富含转录因子，尤其是螺旋-环-螺旋和C2H2型锌指结构域蛋白，这与转录谱的急剧变化相一致。几乎所有在幼小球体中上调的基因在成熟球体中仍表达上调，但少数基因在这两个球体发育阶段有差异表达。成熟小球比年轻小球表达更多的Hsp31和淀粉酶，表达更少的酪氨酸酶。检测到一些转座子的表达，大多数差异表达的转座子在球体中上调。
Coccidioides immitis and C. posadasii are dimorphic fungi that transform from mycelia with internal arthroconidia in the soil to a tissue form known as a spherule in mammals. This process can be recapitulated in vitro by increasing the temperature, CO2 and changing other culture conditions. In this study, we have analyzed changes in gene expression in mycelia and young and mature spherules. Genes that were highly upregulated in young spherules include a spherule surface protein and iron and copper membrane transporters. Genes that are unique to Coccidioides spp. are also overrepresented in this group, suggesting that they may be important for spherule differentiation. Enriched GO terms in young spherule upregulated genes include oxidation-reduction, response to stress and membrane proteins. Downregulated genes are enriched for transcription factors, especially helix–loop–helix and C2H2 type zinc finger domain-containing proteins, which is consistent with the dramatic change in transcriptional profile. Almost all genes that are upregulated in young spherules remain upregulated in mature spherules, but a small number of genes are differentially expressed in those two stages of spherule development. Mature spherules express more Hsp31 and amylase and less tyrosinase than young spherules. Some expression of transposons was detected and most of the differentially expressed transposons were upregulated in spherules.
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DOI：
10.3390/jof4010039
发表时间：
2018-03-20
期刊：
Journal of fungi (Basel, Switzerland)
影响因子：
--
作者：
Basenko EY;Pulman JA;Shanmugasundram A;Harb OS;Crouch K;Starns D;Warrenfeltz S;Aurrecoechea C;Stoeckert CJ Jr;Kissinger JC;Roos DS;Hertz-Fowler C
通讯作者：
Hertz-Fowler C
影响因子：
--
作者：
Hwang, Lena H.;Seth, Erica;Sil, Anita
通讯作者：
Sil, Anita
影响因子：
3.1
作者：
COLE, GT;KIRKLAND, TN;HEARN, VM
通讯作者：
HEARN, VM
DOI：
10.1093/bioinformatics/btu627
发表时间：
2015-02-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Priebe S;Kreisel C;Horn F;Guthke R;Linde J
通讯作者：
Linde J
影响因子：
46.9
作者：
Bray, Nicolas L.;Pimentel, Harold;Pachter, Lior
通讯作者：
Pachter, Lior