A high-quality genome assembly highlights rye genomic characteristics and agronomically important genes.
A high-quality genome assembly highlights rye genomic characteristics and agronomically important genes.
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DOI：
10.1038/s41588-021-00808-z
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发表时间：
2021-04
期刊：
影响因子：
30.8
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Li G;Wang L;Yang J;He H;Jin H;Li X;Ren T;Ren Z;Li F;Han X;Zhao X;Dong L;Li Y;Song Z;Yan Z;Zheng N;Shi C;Wang Z;Yang S;Xiong Z;Zhang M;Sun G;Zheng X;Gou M;Ji C;Du J;Zheng H;Doležel J;Deng XW;Stein N;Yang Q;Zhang K;Wang D
黑麦是一种有价值的食物和饲料作物，是小麦和小黑麦改良的重要遗传资源，也是禾本科植物基因组比较研究的重要材料。在这里，我们对中国黑麦优良品种威宁黑麦的基因组进行了测序。组装的contigs （7.74 Gb）占估计基因组大小（7.86 Gb）的98.47%，其中93.67%的contigs （7.25 Gb）分配给7条染色体。重复元件占组装基因组的90.31%。与先前测序的黑麦科基因组相比，Daniela、Sumaya和Sumana逆转录转座子在黑麦中扩增明显。对威宁组合的进一步分析有助于揭示全基因组基因重复及其对淀粉生物合成基因的影响、复杂蛋白位点的物理组织、早期抽穗性状的基因表达特征以及推测的驯化相关染色体区域和位点。该基因组序列有望加速黑麦及相关谷类作物的基因组和育种研究。通过对威宁黑麦高质量基因组的组装，揭示了基因重复及其对淀粉生物合成基因的影响、早期抽穗性状的基因表达特征和驯化相关染色体区域的推测。
Rye is a valuable food and forage crop, an important genetic resource for wheat and triticale improvement and an indispensable material for efficient comparative genomic studies in grasses. Here, we sequenced the genome of Weining rye, an elite Chinese rye variety. The assembled contigs (7.74 Gb) accounted for 98.47% of the estimated genome size (7.86 Gb), with 93.67% of the contigs (7.25 Gb) assigned to seven chromosomes. Repetitive elements constituted 90.31% of the assembled genome. Compared to previously sequenced Triticeae genomes, Daniela, Sumaya and Sumana retrotransposons showed strong expansion in rye. Further analyses of the Weining assembly shed new light on genome-wide gene duplications and their impact on starch biosynthesis genes, physical organization of complex prolamin loci, gene expression features underlying early heading trait and putative domestication-associated chromosomal regions and loci in rye. This genome sequence promises to accelerate genomic and breeding studies in rye and related cereal crops. A high-quality genome assembly of Weining rye sheds new light on gene duplications and their effects on starch biosynthesis genes, gene expression features underlying early heading trait and putative domestication-associated chromosomal regions.
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影响因子：
64.8
作者：
Luo MC;Gu YQ;Puiu D;Wang H;Twardziok SO;Deal KR;Huo N;Zhu T;Wang L;Wang Y;McGuire PE;Liu S;Long H;Ramasamy RK;Rodriguez JC;Van SL;Yuan L;Wang Z;Xia Z;Xiao L;Anderson OD;Ouyang S;Liang Y;Zimin AV;Pertea G;Qi P;Bennetzen JL;Dai X;Dawson MW;Müller HG;Kugler K;Rivarola-Duarte L;Spannagl M;Mayer KFX;Lu FH;Bevan MW;Leroy P;Li P;You FM;Sun Q;Liu Z;Lyons E;Wicker T;Salzberg SL;Devos KM;Dvořák J
通讯作者：
Dvořák J
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影响因子：
30.8
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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作者：
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通讯作者：
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