Whole-brain imaging of freely-moving zebrafish.
Whole-brain imaging of freely-moving zebrafish.
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DOI：
10.3389/fnins.2023.1127574
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发表时间：
2023
影响因子：
4.3
通讯作者：
Goodhill, Geoffrey J.
中科院分区：
文献类型：
--
作者：
Hasani, Hamid;Sun, Jipeng;Zhu, Shuyu I.;Rong, Qiangzhou;Willomitzer, Florian;Amor, Rumelo;McConnell, Gail;Cossairt, Oliver;Goodhill, Geoffrey J.
神经科学的圣杯之一就是记录动物自由活动和执行复杂行为任务时大脑中每个神经元的活动。虽然最近在啮齿动物模型的大规模神经记录方面取得了重要进展，但整个哺乳动物大脑的单个神经元分辨率仍然难以捉摸。相比之下，斑马鱼的幼虫在这方面提供了很大的希望。斑马鱼是一种脊椎动物模型，与哺乳动物的大脑有很大的同源性，但它们的透明性允许使用光学显微镜技术在单神经元分辨率下记录全脑的遗传编码荧光指标。此外，斑马鱼在很小的时候就开始表现出复杂的自然行为，包括利用视觉线索捕猎小型、快速移动的猎物。直到最近，研究这些行为的神经基础的工作主要依赖于将鱼固定在显微镜物镜下的实验，以及虚拟呈现的猎物等刺激。然而，最近在开发非固定斑马鱼的脑成像技术方面取得了重大进展。在这里，我们讨论了最近的进展，特别关注基于光场显微镜的技术。我们还提请注意几个重要的突出问题，这些问题仍有待解决，以提高所获得结果的生态有效性。
One of the holy grails of neuroscience is to record the activity of every neuron in the brain while an animal moves freely and performs complex behavioral tasks. While important steps forward have been taken recently in large-scale neural recording in rodent models, single neuron resolution across the entire mammalian brain remains elusive. In contrast the larval zebrafish offers great promise in this regard. Zebrafish are a vertebrate model with substantial homology to the mammalian brain, but their transparency allows whole-brain recordings of genetically-encoded fluorescent indicators at single-neuron resolution using optical microscopy techniques. Furthermore zebrafish begin to show a complex repertoire of natural behavior from an early age, including hunting small, fast-moving prey using visual cues. Until recently work to address the neural bases of these behaviors mostly relied on assays where the fish was immobilized under the microscope objective, and stimuli such as prey were presented virtually. However significant progress has recently been made in developing brain imaging techniques for zebrafish which are not immobilized. Here we discuss recent advances, focusing particularly on techniques based on light-field microscopy. We also draw attention to several important outstanding issues which remain to be addressed to increase the ecological validity of the results obtained.
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