Two pleiotropic classes of daf-2 mutation affect larval arrest, adult behavior, reproduction and longevity in Caenorhabditis elegans.
Two pleiotropic classes of daf-2 mutation affect larval arrest, adult behavior, reproduction and longevity in Caenorhabditis elegans.
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daf-2 突变的两类多效性会影响秀丽隐杆线虫的幼虫停滞、成虫行为、繁殖和寿命。
DOI：
10.1093/genetics/150.1.129
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Riddle,DL
中科院分区：
文献类型：
--
作者：
Gems,D;Sutton,AJ;Sundermeyer,ML;Albert,PS;King,KV;Edgley,ML;Larsen,PL;Riddle,DL
线虫秀丽隐杆线虫响应过度拥挤和缺乏食物逮捕发展作为一个dauer幼虫，非喂养，长寿，抗应激，替代第三幼虫阶段。先前的工作已经表明，基因daf-2（编码胰岛素受体家族的一个成员）和age-1（编码PI 3-激酶）中的突变导致组成性形成的dauer幼虫（Daf-c），增加的成体寿命（Age），和增加的内在耐热性（Itt）。一些daf-2突变体具有额外的发育、行为和生殖缺陷。我们详细描述了15个温度敏感型和1个非条件性daf-2等位基因，以研究daf-2突变缺陷的程度，并检查特定突变性状是否相互关联。在daf-2突变体中观察到的最长寿命大约是野生型的三倍。温度敏感的daf-2突变体分为两个重叠的类别，包括8个1类突变体，它们是Daf-c，Age和Itt，并在25.5°下表现出低水平的L1阻滞。7个2类突变体也表现出1类缺陷以及以下部分或全部：减少成年运动，异常的成年体和性腺形态，高水平的胚胎和L1停滞，生产的后代在生命后期，和减少育雏大小。Daf-c、年龄和Itt表型的强度在很大程度上彼此相关，但与2类特异性缺陷的强度无关。这表明β 2受体是双功能的。空表型的检查显示了一个母亲拯救的鸡蛋，L1致死成分，和一个非条件性的Daf-c成分。对于Daf-c表型，dauer缺陷型（Daf-d）突变daf-12（m20）对daf-2 1类等位基因具有上位性，但对检测的严重2类等位基因则无上位性。daf-d突变daf-16（m26）抑制了所有daf-2突变缺陷。我们的发现为daf-2、age-1、daf-12和daf-16的相互作用提供了一个新的模型。
The nematode Caenorhabditis elegans responds to overcrowding and scarcity of food by arresting development as a dauer larva, a nonfeeding, long-lived, stress-resistant, alternative third-larval stage. Previous work has shown that mutations in the genes daf-2 (encoding a member of the insulin receptor family) and age-1 (encoding a PI 3-kinase) result in constitutive formation of dauer larvae (Daf-c), increased adult longevity (Age), and increased intrinsic thermotolerance (Itt). Some daf-2 mutants have additional developmental, behavioral, and reproductive defects. We have characterized in detail 15 temperature-sensitive and 1 nonconditional daf-2 allele to investigate the extent of daf-2 mutant defects and to examine whether specific mutant traits correlate with each other. The greatest longevity seen in daf-2 mutant adults was approximately three times that of wild type. The temperature-sensitive daf-2 mutants fell into two overlapping classes, including eight class 1 mutants, which are Daf-c, Age, and Itt, and exhibit low levels of L1 arrest at 25.5°. Seven class 2 mutants also exhibit the class 1 defects as well as some or all of the following: reduced adult motility, abnormal adult body and gonad morphology, high levels of embryonic and L1 arrest, production of progeny late in life, and reduced brood size. The strengths of the Daf-c, Age, and Itt phenotypes largely correlated with each other but not with the strength of class 2-specific defects. This suggests that the DAF-2 receptor is bifunctional. Examination of the null phenotype revealed a maternally rescued egg, L1 lethal component, and a nonconditional Daf-c component. With respect to the Daf-c phenotype, the dauer-defective (Daf-d) mutation daf-12(m20) was epistatic to daf-2 class 1 alleles but not the severe class 2 alleles tested. All daf-2 mutant defects were suppressed by the daf-d mutation daf-16(m26). Our findings suggest a new model for daf-2, age-1, daf-12, and daf-16 interactions.
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影响因子：
64.8
作者：
Morris, JZ;Tissenbaum, HA;Ruvkun, G
通讯作者：
Ruvkun, G
影响因子：
3.3
作者：
D. Friedman;T. Johnson
通讯作者：
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美洲蟑螂 (Periplaneta americana L.) 的繁殖力、寿命和单性生殖。
DOI：
--
发表时间：
1942
期刊：
Physiological Zoology
影响因子：
--
作者：
J. T. Griffiths;O. E. Tauber
通讯作者：
O. E. Tauber
影响因子：
3.3
作者：
P. L. Larsen;P. Albert;D. Riddle
通讯作者：
P. L. Larsen;P. Albert;D. Riddle
影响因子：
4.6
作者：
Adam Antebi;J. Culotti;E. Hedgecock
通讯作者：
Adam Antebi;J. Culotti;E. Hedgecock