Alterations of gut microbial pathways and virulence factors in hemodialysis patients.
Alterations of gut microbial pathways and virulence factors in hemodialysis patients.
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DOI：
10.3389/fcimb.2022.904284
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发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
--
作者：
肠道菌群的改变可能导致终末期肾病患者的尿毒症毒性和免疫失调。血液透析患者容易感染，败血症后死亡率较高。肠道宏基因组中的毒力因子尚未在血液透析患者中得到很好的研究，这些毒力因子可以被微生物利用以在其宿主中成功地茁壮成长。在这项研究中，我们对16名对照受试者和24名血液透析患者的粪便DNA进行了鸟枪宏基因组学测序。我们的分析表明，与对照组相比，血液透析患者中的许多微生物种类、代谢途径、抗生素耐药性和毒力因子发生了显著改变。特别是，红霉素耐药性甲基化酶，吡哆胺5-磷酸氧化酶，和链丝菌素乙酰转移酶显着增加血液透析患者。我们的研究结果为进一步阐明毒力因子在HD患者感染易感性中的致病作用和制定治疗策略以降低HD患者抗生素耐药性和毒力因子的遗传能力奠定了有价值的基础。
Alterations in gut microbiota might contribute to uremic toxicity and immune dysregulation in patients with end-stage renal disease. Hemodialysis patients are prone to infection and higher mortality following sepsis. The virulence factors in the gut metagenome have not been well studied in hemodialysis patients, which could be employed by microorganisms to successfully thrive and flourish in their hosts. In this study, we performed shotgun metagenomics sequencing on fecal DNA collected from 16 control subjects and 24 hemodialysis patients. Our analysis shows that a number of microbial species, metabolic pathways, antibiotic resistance, and virulence factors were significantly altered in hemodialysis patients compared with controls. In particular, erythromycin resistance methylase, pyridoxamine 5-phosphate oxidase, and streptothricin-acetyl-transferase were significantly increased in hemodialysis patients. The findings in our study laid a valuable foundation to further elucidate the causative role of virulence factors in predisposing HD patients to infection and to develop treatment strategies to reduce the genetic capacities of antibiotic resistance and virulence factors in HD patients.
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影响因子：
2.3
作者：
Kambhampati, Ganesh;Asmar, Abdo;Ejaz, A. Ahsan
通讯作者：
Ejaz, A. Ahsan
影响因子：
48
作者：
Segata, Nicola;Waldron, Levi;Ballarini, Annalisa;Narasimhan, Vagheesh;Jousson, Olivier;Huttenhower, Curtis
通讯作者：
Huttenhower, Curtis
影响因子：
4.3
作者：
Kaminski J;Gibson MK;Franzosa EA;Segata N;Dantas G;Huttenhower C
通讯作者：
Huttenhower C
影响因子：
6
作者：
Gao B;Friedman ES;Regunathan R;Shrivastava A;Barrows IR;Amdur RL;Andrews SC;Barrows ED;Raj DS
通讯作者：
Raj DS
影响因子：
24.5
作者：
Joossens, Marie;Huys, Geert;Vermeire, Severine
通讯作者：
Vermeire, Severine