A plant germline-specific integrator of sperm specification and cell cycle progression.
A plant germline-specific integrator of sperm specification and cell cycle progression.
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DOI：
10.1371/journal.pgen.1000430
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发表时间：
2009-03
期刊：
影响因子：
4.5
通讯作者：
Twell D
中科院分区：
文献类型：
--
作者：
Brownfield L;Hafidh S;Borg M;Sidorova A;Mori T;Twell D
开花植物独特的双受精机制依赖于一对功能正常的精子细胞。在雄配子发生过程中，每个单倍体小孢子经历不对称分裂，产生一个大的、非生殖系的营养细胞和一个单独的生殖细胞，该细胞分裂一次以产生精子对。尽管精子在植物繁殖中很重要，但对控制生殖细胞增殖和规格的分子机制知之甚少。在这里，我们研究了拟南芥雄性生殖系特异性Myb蛋白Duo POLLEN1，DUO1作为雄性生殖系发育的正调控因子的作用。我们证明DUO1是正确的雄性生殖细胞分化所必需的，包括受精所需的关键基因的表达。Duo1也是雄性生殖细胞分裂所必需的，我们证明了Duo1是G2/M调节因子AtCycB1；1的胚系表达所必需的，并且AtCycB1：1可以部分挽救Duo1中有缺陷的生殖细胞分裂。我们进一步证明，DUO1的雄性生殖系限制性表达依赖于正的启动子元件，而不是所建议的抑制物结合部位。因此，DUO1是开花植物生产功能性精子细胞的关键调控因子，具有连接配子细胞规格和细胞周期进程的新的整合作用。与动物不同，开花植物需要的不是一个而是两个精子细胞才能成功受精--一个精子细胞与卵细胞结合产生胚胎，另一个精子细胞与中央细胞结合产生种子内营养丰富的胚乳组织。这一“双受精”过程中的一个谜团是，每一粒花粉如何产生生育和种子生产所需的一对精子细胞。在这里，我们报告了DUO1的双重作用的发现，DUO1是植物精子细胞产生所需的调节基因。我们发现，DUO1基因是促进精子前体细胞分裂所必需的，同时也促进其分化为有功能的精子细胞。Duo1是表达关键的细胞周期调节因子和表达对配子分化和受精至关重要的基因所必需的。这项工作首次提供了对开花植物细胞周期进程和配子分化协调机制的分子洞察。这些知识将有助于理解开花植物配子发育的机制和进化，并可能有助于控制基因流动和杂交行为。
The unique double fertilisation mechanism in flowering plants depends upon a pair of functional sperm cells. During male gametogenesis, each haploid microspore undergoes an asymmetric division to produce a large, non-germline vegetative cell and a single germ cell that divides once to produce the sperm cell pair. Despite the importance of sperm cells in plant reproduction, relatively little is known about the molecular mechanisms controlling germ cell proliferation and specification. Here, we investigate the role of the Arabidopsis male germline-specific Myb protein DUO POLLEN1, DUO1, as a positive regulator of male germline development. We show that DUO1 is required for correct male germ cell differentiation including the expression of key genes required for fertilisation. DUO1 is also necessary for male germ cell division, and we show that DUO1 is required for the germline expression of the G2/M regulator AtCycB1;1 and that AtCycB1:1 can partially rescue defective germ cell division in duo1. We further show that the male germline-restricted expression of DUO1 depends upon positive promoter elements and not upon a proposed repressor binding site. Thus, DUO1 is a key regulator in the production of functional sperm cells in flowering plants that has a novel integrative role linking gametic cell specification and cell cycle progression. Flowering plants, unlike animals, require not one, but two sperm cells for successful fertilisation—one sperm cell to join with the egg cell to produce the embryo and the other to join with the central cell to produce the nutrient-rich endosperm tissue inside the seed. A mystery in this “double fertilization” process was how each single pollen grain could produce the pair of sperm cells needed for fertility and seed production. Here, we report the discovery of a dual role for DUO1, a regulatory gene required for plant sperm cell production. We show that the DUO1 gene is required to promote the division of sperm precursor cells, while at the same time promoting their differentiation into functional sperm cells. DUO1 is required for the expression of a key cell cycle regulator and for the expression of genes that are critical for gamete differentiation and fertilisation. This work provides the first molecular insight into the mechanisms through which cell cycle progression and gamete differentiation are coordinated in flowering plants. This knowledge will be helpful in understanding the mechanisms and evolution of gamete development in flowering plants and may be useful in the control of gene flow and crossing behaviour.
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影响因子：
9.2
作者：
Hirai, Makoto;Arai, Meiji;Matsuoka, Hiroyuki
通讯作者：
Matsuoka, Hiroyuki
影响因子：
7.2
作者：
Colón-Carmona, A;You, R;Doerner, P
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影响因子：
12.3
作者：
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作者：
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鉴定拟南芥在微汞生成的早期阶段靶向操纵基因表达的小孢子活性启动子。
影响因子：
5.3
作者：
Honys D;Oh SA;Renák D;Donders M;Solcová B;Johnson JA;Boudová R;Twell D
通讯作者：
Twell D