Assignment of Atlantic salmon (Salmo salar) linkage groups to specific chromosomes: conservation of large syntenic blocks corresponding to whole chromosome arms in rainbow trout (Oncorhynchus mykiss).
Assignment of Atlantic salmon (Salmo salar) linkage groups to specific chromosomes: conservation of large syntenic blocks corresponding to whole chromosome arms in rainbow trout (Oncorhynchus mykiss).
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DOI：
10.1186/1471-2156-10-46
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发表时间：
2009-08-18
期刊：
影响因子：
2.9
通讯作者：
Davidson WS
中科院分区：
文献类型：
--
作者：
Phillips RB;Keatley KA;Morasch MR;Ventura AB;Lubieniecki KP;Koop BF;Danzmann RG;Davidson WS
大多数硬骨鱼物种，尤其是淡水类群，例如鲑科鱼的近亲，具有由 25 对近端着丝粒染色体和 48-52 条染色体臂组成的核型。鲑鱼的共同祖先经历了全基因组复制后，其核型将具有 100 个染色体臂，这反映在现存鲑鱼（例如虹鳟鱼）中看到的 96-104 条染色体臂的众数范围中。大西洋鲑鱼是鲑鱼中的一个例外，因为它有 72-74 条染色体臂，其核型包括 12 对大的近端着丝粒染色体，这似乎是串联融合的结果。本研究的目的是整合大西洋鲑鱼的连锁图和核型，并将染色体图与虹鳟鱼的染色体图进行比较。使用含有映射到每个连锁群的遗传标记的 BAC 探针进行荧光原位杂交，将大西洋鲑鱼遗传连锁群分配给欧洲亚种的特定染色体。与相似大小的近端着丝粒染色体相比，中着丝粒染色体的遗传连锁群更大。大西洋鲑鱼染色体图谱与虹鳟鱼染色体图谱的比较为这些物种中大同线性块的保存提供了强有力的证据，对应于虹鳟鱼的整个染色体臂。有人认为，大西洋鲑鱼的一些大的近端着丝粒染色体是串联融合的结果，臂中部的小块重复DNA代表染色体融合的位点。较大的近端着丝粒染色体内重复 DNA 块两侧的染色体区域对应于不同的虹鳟鱼染色体臂，这一发现为这一假设提供了支持。
Most teleost species, especially freshwater groups such as the Esocidae which are the closest relatives of salmonids, have a karyotype comprising 25 pairs of acrocentric chromosomes and 48–52 chromosome arms. After the common ancestor of salmonids underwent a whole genome duplication, its karyotype would have 100 chromosome arms, and this is reflected in the modal range of 96–104 seen in extant salmonids (e.g., rainbow trout). The Atlantic salmon is an exception among the salmonids as it has 72–74 chromosome arms and its karyotype includes 12 pairs of large acrocentric chromosomes, which appear to be the result of tandem fusions. The purpose of this study was to integrate the Atlantic salmon's linkage map and karyotype and to compare the chromosome map with that of rainbow trout. The Atlantic salmon genetic linkage groups were assigned to specific chromosomes in the European subspecies using fluorescence in situ hybridization with BAC probes containing genetic markers mapped to each linkage group. The genetic linkage groups were larger for metacentric chromosomes compared to acrocentric chromosomes of similar size. Comparison of the Atlantic salmon chromosome map with that of rainbow trout provides strong evidence for conservation of large syntenic blocks in these species, corresponding to entire chromosome arms in the rainbow trout. It had been suggested that some of the large acrocentric chromosomes in Atlantic salmon are the result of tandem fusions, and that the small blocks of repetitive DNA in the middle of the arms represent the sites of chromosome fusions. The finding that the chromosomal regions on either side of the blocks of repetitive DNA within the larger acrocentric chromosomes correspond to different rainbow trout chromosome arms provides support for this hypothesis.
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影响因子：
4.4
作者：
Guyomard, Rene;Mauger, Stephane;Tabet-Canale, Kamila;Martineau, Sylvain;Genet, Carine;Krieg, Francine;Quillet, Edwige
通讯作者：
Quillet, Edwige
影响因子：
1.7
作者：
Artieri, CG;Mitchell, LA;Davidson, WS
通讯作者：
Davidson, WS
影响因子：
3.1
作者：
Danzmann, RG;Cairney, M;Woram, RA
通讯作者：
Woram, RA
影响因子：
4.4
作者：
Moen, Thomas;Hayes, Ben;Baranski, Matthew;Berg, Paul R.;Kjoglum, Sissel;Koop, Ben F.;Davidson, Willie S.;Omholt, Stig W.;Lien, Sigbjorn
通讯作者：
Lien, Sigbjorn
影响因子：
4.4
作者：
Lukacs MF;Harstad H;Grimholt U;Beetz-Sargent M;Cooper GA;Reid L;Bakke HG;Phillips RB;Miller KM;Davidson WS;Koop BF
通讯作者：
Koop BF