The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo.
The role of endothelial cells in myofiber differentiation and the vascularization and innervation of bioengineered muscle tissue in vivo.
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DOI：
10.1016/j.biomaterials.2012.09.045
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发表时间：
2013-01
期刊：
影响因子：
14
通讯作者：
Soker, Shay
中科院分区：
文献类型：
--
作者：
Criswell, Tracy L.;Corona, Benjamin T.;Wang, Zhan;Zhou, Yu;Niu, Guoguang;Xu, Yong;Christ, George J.;Soker, Shay
肌肉骨骼疾病是致残的主要原因，目前缺乏有效的治疗方法。组织工程提供了利用细胞和生物材料来恢复肌肉体积和功能的新疗法的可能性。骨骼肌工程中的一个主要考虑因素是在再生组织中整合功能性血管系统。在这项研究中，我们使用荧光细胞标记来跟踪共培养细胞在体内和体外的位置和分化。我们首先利用荧光标记内皮细胞（ECs）和肌肉祖细胞（MPCs）的共培养，在生物工程肌肉的体内模型中研究ECs促进肌肉组织形成和血管化的能力。与单独植入MPCs的支架相比，同时植入MPCs和ECs的支架血管化、组织形成和神经支配明显增强。随后，我们使用3细胞类型系统（ECs， MPCs和周细胞（PCs））进行了体外实验，以证明荧光细胞标记在监测细胞生长和分化方面的效用。单个细胞类型的生长和分化是用活细胞荧光显微镜确定的，展示了荧光标记实时监测组织组织的效用。
Musculoskeletal disorders are a major cause of disability and effective treatments are currently lacking. Tissue engineering affords the possibility of new therapies utilizing cells and biomaterials for the recovery of muscle volume and function. A major consideration in skeletal muscle engineering is the integration of a functional vasculature within the regenerating tissue. In this study we employed fluorescent cell labels to track the location and differentiation of co-cultured cells in vivo and in vitro. We first utilized a co-culture of fluorescently labeled endothelial cells (ECs) and muscle progenitor cells (MPCs) to investigate the ability of ECs to enhance muscle tissue formation and vascularization in an in vivo model of bioengineered muscle. Scaffolds that had been seeded with both MPCs and ECs showed significantly greater vascularization, tissue formation and enhanced innervation as compared to scaffolds seeded with MPCs alone. Subsequently, we performed in vitro experiments using a 3-cell type system (ECs, MPCs, and pericytes (PCs)) to demonstrate the utility of fluorescent cell labeling for monitoring cell growth and differentiation. The growth and differentiation of individual cell types was determined using live cell fluorescent microscopy demonstrating the utility of fluorescent labels to monitor tissue organization in real time.
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影响因子：
4.8
作者：
Golini, Lucia;Chouabe, Christophe;Sorrentino, Vincenzo
通讯作者：
Sorrentino, Vincenzo
影响因子：
3.6
作者：
Borschel, GH;Dennis, RG;Kuzon, WM
通讯作者：
Kuzon, WM
血管细胞对成肌干细胞行为的调控 卫星细胞、内皮周围细胞和内皮细胞的 "三人行"
影响因子：
4.3
作者：
Abou-Khalil, Rana;Mounier, Remi;Chazaud, Benedicte
通讯作者：
Chazaud, Benedicte
影响因子：
--
作者：
De Coppi, Paolo;Bellini, Silvia;Paolo Parnigotto, Pier
通讯作者：
Paolo Parnigotto, Pier
DOI：
10.1073/pnas.182296499
发表时间：
2002-09-03
影响因子：
11.1
作者：
Jin, KL;Zhu, YH;Greenberg, DA
通讯作者：
Greenberg, DA