Excitatory and inhibitory synaptic function in the rostral nucleus of the solitary tract in embryonic rat.
Excitatory and inhibitory synaptic function in the rostral nucleus of the solitary tract in embryonic rat.
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DOI：
10.1016/j.brainres.2012.10.037
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发表时间：
2013-01-15
期刊：
影响因子：
2.9
通讯作者：
Bradley RM
中科院分区：
文献类型：
--
作者：
Suwabe T;Mistretta CM;Bradley RM
本实验观察了大鼠孤束嘴侧核（rNST）内突触的胚胎发育过程，以确定突触开始发挥功能的时间。使用脑片制备，我们研究了突触受体的出现在二级rNST神经元和调查的发展引起的传入神经刺激突触后反应。产前兴奋性和抑制性突触反应，早在E14。早在E16就检测到谷氨酸能和GABA能突触后反应。NMDA和AMPA受体都参与了突触后兴奋反应。GABA能突触后反应主要由GABAA受体的激活引起。然而，功能性GABAC受体也被证明。一个甘氨酸能突触后反应没有发现，虽然功能性甘氨酸受体证明在E16。孤束（ST）刺激诱发的EPSC，首先在E16检测到，消除谷氨酸受体拮抗剂。ST-诱发的IPSPs，也检测到在E16，消除GABAA受体拮抗剂。因此，相当多的产前发展的rNST突触连接发生，这将确保出生后中枢味觉处理电路的功能。
The embryonic development of synapses in the rostral nucleus of the solitary tract (rNST) was investigated in rat to determine when synapses begin to function. Using a brain slice preparation we studied appearance of synaptic receptors on second order rNST neurons and investigated the development of postsynaptic responses elicited by afferent nerve stimulation. Prenatal excitatory and inhibitory synaptic responses were recorded as early as E14. Glutamatergic and GABAergic postsynaptic responses were detected as early as E16. Both NMDA and AMPA receptors contributed to glutamatergic postsynaptic responses. GABAergic postsynaptic responses resulted primarily from activation of GABAA receptors. However, functional GABAC receptors were also demonstrated. A glycinergic postsynaptic response was not found although functional glycine receptors were demonstrated at E16. Solitary tract (ST) stimulation -evoked EPSCs, first detected at E16, were eliminated by glutamate receptor antagonists. ST-evoked IPSPs, also detected at E16, were eliminated by GABAA receptor antagonist. Thus, considerable prenatal development of rNST synaptic connections occurs and this will ensure postnatal function of central taste processing circuits.
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影响因子：
5.5
作者：
Dufour, Amandine;Tell, Fabien;Baude, Agnes
通讯作者：
Baude, Agnes
影响因子：
2.5
作者：
HILL, DL;BRADLEY, RM;MISTRETTA, CM
通讯作者：
MISTRETTA, CM
影响因子：
2.5
作者：
KING, CT;HILL, DL
通讯作者：
HILL, DL
影响因子：
3.5
作者：
Du, JH;Bradley, RM
通讯作者：
Bradley, RM
DOI：
10.1016/s0165-3806(02)00490-x
发表时间：
2002-10-20
期刊：
DEVELOPMENTAL BRAIN RESEARCH
影响因子：
--
作者：
Kihara, M;Yoshioka, H;Sawada, T
通讯作者：
Sawada, T