Genome-wide association study reveals novel players in defense hormone crosstalk in Arabidopsis.
Genome-wide association study reveals novel players in defense hormone crosstalk in Arabidopsis.
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DOI：
10.1111/pce.13357
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发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Pieterse CMJ
中科院分区：
文献类型：
--
作者：
Proietti S;Caarls L;Coolen S;Van Pelt JA;Van Wees SCM;Pieterse CMJ
茉莉酸（JA）调节植物对坏死性病原体和昆虫食草动物的防御。水杨酸（SA）和脱落酸（阿坝）可以拮抗JA调节的防御，从而调节病原体或昆虫的抗性。我们对拟南芥的自然遗传变异进行了全基因组关联（GWA）研究，以了解SA和阿坝对JA途径的影响。我们用甲基JA（MeJA）或MeJA与SA或阿坝的组合处理349份拟南芥属种质，之后将JA响应标记基因PLANT DEFENSIN1.2（PDF1.2）的表达定量作为GWA分析的读数。这两种激素拮抗MeJA诱导的PDF1.2在大多数的加入，但在幅度上有很大的变化。SA和阿坝影响的PDF1.2表达数据的GWA作图揭示了与串扰相关的位点。通过T-DNA插入突变体分析证实GLYI 4（编码glycoprotein酶）和ARR 11（编码参与细胞分裂素信号传导的拟南芥反应调节剂）影响SA-JA串扰和对坏死营养型灰葡萄孢的抗性。此外，At 1g 16310（编码阳离子外排家族蛋白）被证实影响ABA-JA串扰和对甘蓝夜蛾草食性的易感性。总的来说，这项GWA研究确定了JA激素串扰中的新参与者，这些参与者在病原体或昆虫抗性的调节中具有潜在作用。防御相关激素之间的串扰使植物能够微调它们对环境中遇到的过多攻击者的免疫反应。我们挖掘了拟南芥中的天然遗传变异，以寻找与应激激素水杨酸和脱落酸对茉莉酸调节的防御反应的积极或消极相互作用相关的新基因。通过全基因组关联作图，我们确定了三个在激素串扰中具有假定作用的新基因，这些基因影响了对坏死营养型病原体灰葡萄孢菌或昆虫食草动物甘蓝夜蛾的抗性水平。
Jasmonic acid (JA) regulates plant defenses against necrotrophic pathogens and insect herbivores. Salicylic acid (SA) and abscisic acid (ABA) can antagonize JA‐regulated defenses, thereby modulating pathogen or insect resistance. We performed a genome‐wide association (GWA) study on natural genetic variation in Arabidopsis thaliana for the effect of SA and ABA on the JA pathway. We treated 349 Arabidopsis accessions with methyl JA (MeJA), or a combination of MeJA and either SA or ABA, after which expression of the JA‐responsive marker gene PLANT DEFENSIN1.2 (PDF1.2) was quantified as a readout for GWA analysis. Both hormones antagonized MeJA‐induced PDF1.2 in the majority of the accessions but with a large variation in magnitude. GWA mapping of the SA‐ and ABA‐affected PDF1.2 expression data revealed loci associated with crosstalk. GLYI4 (encoding a glyoxalase) and ARR11 (encoding an Arabidopsis response regulator involved in cytokinin signalling) were confirmed by T‐DNA insertion mutant analysis to affect SA–JA crosstalk and resistance against the necrotroph Botrytis cinerea. In addition, At1g16310 (encoding a cation efflux family protein) was confirmed to affect ABA–JA crosstalk and susceptibility to Mamestra brassicae herbivory. Collectively, this GWA study identified novel players in JA hormone crosstalk with potential roles in the regulation of pathogen or insect resistance. Crosstalk between defense‐related hormones enables plants to finely tune their immune response to the plethora of attackers that they encounter in their environment. We mined the natural genetic variation in Arabidopsis thaliana for novel genes that are associated with positive or negative interactions that the stress hormones salicylic acid and abscisic acid have on jasmonic acid‐regulated defense responses. Through genome‐wide association mapping, we identified three novel genes with putative roles in hormone crosstalk and that impact the level of resistance against the necrotrophic pathogen Botrytis cinerea or the insect herbivore Mamestra brassicae.
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影响因子：
4.5
作者：
Baxter I;Brazelton JN;Yu D;Huang YS;Lahner B;Yakubova E;Li Y;Bergelson J;Borevitz JO;Nordborg M;Vitek O;Salt DE
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