Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.
Ice Inhibition for Cryopreservation: Materials, Strategies, and Challenges.
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冷冻保存的冰抑制：材料、策略和挑战。
DOI：
10.1002/advs.202002425
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发表时间：
2021-03
期刊：
影响因子：
--
通讯作者：
Zhao G
中科院分区：
文献类型：
--
作者：
Chang T;Zhao G
冷冻保存技术已发展成为生物医学应用的基本和重要的支持方法，如基于细胞的治疗，组织工程，辅助生殖和疫苗储存。冰晶的形成、生长和重结晶是细胞/组织/器官低温保存的主要限制因素，对低温保存的生物样品造成致命的冷冻损伤。蓬勃发展的防冰材料和策略可以有效地调节和抑制冰晶，从而减少冰损伤，提高低温保存效率。本文首先介绍了低温保存过程中冰损伤的基本机制。综述了近年来化学抑冰分子的研究进展，包括低温保护剂、抗冻蛋白、合成高分子、纳米材料和水凝胶等，并介绍了它们在低温保存中的应用。进一步讨论了先进的工程策略，包括海藻糖递送、细胞包封和用于冰抑制的仿生结构设计。此外，还系统地综述了用于抑制冷却和解冻过程中冰晶的外物理场技术。最后，目前的挑战和未来的前景，在该领域的冰抑制高效冷冻保存提出。本文介绍了低温保存过程中冰损伤的基本机制，并介绍了最新的冰抑制材料和策略，包括冷却和解冻过程，以实现高效的低温保存。展望了未来的发展前景和面临的挑战，以促进细胞、组织和器官冷冻保存的发展，并为低温生物学提供新的灵感。
Cryopreservation technology has developed into a fundamental and important supporting method for biomedical applications such as cell‐based therapeutics, tissue engineering, assisted reproduction, and vaccine storage. The formation, growth, and recrystallization of ice crystals are the major limitations in cell/tissue/organ cryopreservation, and cause fatal cryoinjury to cryopreserved biological samples. Flourishing anti‐icing materials and strategies can effectively regulate and suppress ice crystals, thus reducing ice damage and promoting cryopreservation efficiency. This review first describes the basic ice cryodamage mechanisms in the cryopreservation process. The recent development of chemical ice‐inhibition molecules, including cryoprotectant, antifreeze protein, synthetic polymer, nanomaterial, and hydrogel, and their applications in cryopreservation are summarized. The advanced engineering strategies, including trehalose delivery, cell encapsulation, and bioinspired structure design for ice inhibition, are further discussed. Furthermore, external physical field technologies used for inhibiting ice crystals in both the cooling and thawing processes are systematically reviewed. Finally, the current challenges and future perspectives in the field of ice inhibition for high‐efficiency cryopreservation are proposed. This work describes the fundamental mechanisms of ice injury during cryopreservation, and introduces the state‐of‐the‐art ice‐inhibition materials and strategies, both in the cooling and thawing processes, for high‐efficiency cryopreservation. Future perspectives and challenges are also proposed to motivate the development of cell, tissue, and organ cryopreservation and offer bright new inspiration for cryobiology.
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影响因子：
16.6
作者：
Biggs CI;Bailey TL;Ben Graham;Stubbs C;Fayter A;Gibson MI
通讯作者：
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影响因子：
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DOI：
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发表时间：
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影响因子：
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通讯作者：
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DOI：
10.1073/pnas.89.19.8953
发表时间：
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影响因子：
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作者：
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通讯作者：
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