Demonstration of cytotoxicity against wasps by pierisin-1: a possible defense factor in the cabbage white butterfly.
Demonstration of cytotoxicity against wasps by pierisin-1: a possible defense factor in the cabbage white butterfly.
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DOI：
10.1371/journal.pone.0060539
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Wakabayashi K
中科院分区：
文献类型：
--
作者：
Takahashi-Nakaguchi A;Matsumoto Y;Yamamoto M;Iwabuchi K;Totsuka Y;Sugimura T;Wakabayashi K
卷心菜白蝴蝶（Pieris rapae）产生一种诱导哺乳动物细胞凋亡的蛋白——pierisin-1。本研究考察了pierisin-1作为一种抗油菜寄生蜂的保护剂的生物活性。Pierisin-1在1 ~ 100µg/ml水平上对菜青虫、白斑斑绒蚧、卡里绒蚧和菜绒蚧的卵和幼虫产生有害影响，与菜青虫幼虫的水平基本相当。与此相反，天然油菜寄生蜂Cotesia glomerata的卵和幼虫通过抑制pierisin-1在表层的渗透来抵抗pierisin-1的毒性。菜青虫寄生后，菜青虫幼虫中pierisin-1 mRNA的表达量升高，而被菜青虫寄生后，其表达量降低。此外，菜蛾与血淋巴中活化的pierisin-1升高有关。综上所述，pierisin-1可能作为一种防御因子，在油菜中起到一定的防御作用。
The cabbage white butterfly, Pieris rapae, produces pierisin-1, a protein inducing apoptosis of mammalian cells. In the present study, the biological activity of pierisin-1 as a protective agent against parasitic wasps for P. rapae was examined. Pierisin-1 caused detrimental effects on eggs and larvae of non-habitual parasitoids for P. rapae, Glyptapanteles pallipes, Cotesia kariyai and Cotesia plutellae at 1–100 µg/ml, levels essentially equivalent to those found in P. rapae larvae. In contrast, eggs and larvae of the natural parasitoid of P. rapae, Cotesia glomerata proved resistant to the toxicity of pierisin-1 through inhibition of pierisin-1 penetration of the surface layer. The expression level of pierisin-1 mRNA in the larvae of P. rapae was increased by parasitization by C. plutellae, whereas it was decreased by C. glomerata. In addition, C. plutellae was associated with elevation of activated pierisin-1 in the hemolymph. From these observations, it is suggested that pierisin-1 could contribute as a defense factor against parasitization by some type of wasps in P. rapae.
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影响因子：
2.6
作者：
Hayakawa, Yoichi;Yazaki, K.;Tanaka, T.
通讯作者：
Tanaka, T.
影响因子：
56.9
作者：
KAYA, HK
通讯作者：
KAYA, HK
影响因子：
2.2
作者：
KITANO, H
通讯作者：
KITANO, H
DOI：
10.1073/pnas.0712221105
发表时间：
2008-02-19
影响因子：
11.1
作者：
Matsumoto, Yasuko;Nakano, Tsuyoshi;Wakabayashi, Keiji
通讯作者：
Wakabayashi, Keiji
影响因子：
2.9
作者：
Natori, S;Shiraishi, H;Kobayashi, A
通讯作者：
Kobayashi, A