Dynamics of responses in compatible potato-Potato virus Y interaction are modulated by salicylic acid.
Dynamics of responses in compatible potato-Potato virus Y interaction are modulated by salicylic acid.
复制标题
DOI：
10.1371/journal.pone.0029009
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Gruden K
中科院分区：
文献类型：
--
作者：
Baebler Š;Stare K;Kovač M;Blejec A;Prezelj N;Stare T;Kogovšek P;Pompe-Novak M;Rosahl S;Ravnikar M;Gruden K
为了研究马铃薯-马铃薯Y病毒(PVY)亲和互作与水杨酸调控途径的关系，我们利用非转基因马铃薯品种进行了实验。Désirée，转基因NahG-Désirée，cv.IgOR和PVYNTN，PVY最具侵袭性的菌株。水杨酸在病毒增殖和症状发展中的重要性得到了证实，在NahG-Désirée出现了明显的症状，水杨酸被耗尽，喷洒2，6-二氯异烟酸(一种水杨酸类似物)后效果逆转。我们已经使用定量聚合酶链式反应来监测病毒的繁殖，以及通过选择光合作用活性、碳水化合物代谢和防御反应的标记基因的表达来监测植物的反应。接种Cv.马铃薯的病毒增殖最慢。Désirée，这是研究中唯一没有症状的基因。防御相关基因在两个敏感基因(NahG-Désirée和cv)中的表达强度要强得多。Igor)，而不是在无症状植株(cv.Désirée)。光合作用和碳水化合物代谢基因的表达在有症状和无症状的表型之间存在差异。这两个敏感基因的差异表达模式表明，相互作用的结果不是简单地依赖于一个调控成分，而是分子水平上不同的反应可以产生相似的表型特征。
To investigate the dynamics of the potato – Potato virus Y (PVY) compatible interaction in relation to salicylic acid - controlled pathways we performed experiments using non-transgenic potato cv. Désirée, transgenic NahG-Désirée, cv. Igor and PVYNTN, the most aggressive strain of PVY. The importance of salicylic acid in viral multiplication and symptom development was confirmed by pronounced symptom development in NahG-Désirée, depleted in salicylic acid, and reversion of the effect after spraying with 2,6-dichloroisonicotinic acid (a salicylic acid - analogue). We have employed quantitative PCR for monitoring virus multiplication, as well as plant responses through expression of selected marker genes of photosynthetic activity, carbohydrate metabolism and the defence response. Viral multiplication was the slowest in inoculated potato of cv. Désirée, the only asymptomatic genotype in the study. The intensity of defence-related gene expression was much stronger in both sensitive genotypes (NahG-Désirée and cv. Igor) at the site of inoculation than in asymptomatic plants (cv. Désirée). Photosynthesis and carbohydrate metabolism gene expression differed between the symptomatic and asymptomatic phenotypes. The differential gene expression pattern of the two sensitive genotypes indicates that the outcome of the interaction does not rely simply on one regulatory component, but similar phenotypical features can result from distinct responses at the molecular level.
登录
查看更多内容
影响因子：
4.4
作者：
Babu M;Griffiths JS;Huang TS;Wang A
通讯作者：
Wang A
影响因子：
4.4
作者：
Hren M;Nikolić P;Rotter A;Blejec A;Terrier N;Ravnikar M;Dermastia M;Gruden K
通讯作者：
Gruden K
影响因子：
3.2
作者：
COQUOZ, JL;BUCHALA, AJ;METRAUX, JP
通讯作者：
METRAUX, JP
影响因子：
6.5
作者：
Milavec, M;Ravnikar, M;Kovac, M
通讯作者：
Kovac, M
影响因子：
2.7
作者：
Navarre, DA;Mayo, D
通讯作者：
Mayo, D